硫醇酯连接的对香豆酸作为一种具有视紫红质样光化学性质的蛋白质中的新型光活性辅基。
Thiol ester-linked p-coumaric acid as a new photoactive prosthetic group in a protein with rhodopsin-like photochemistry.
作者信息
Hoff W D, Düx P, Hård K, Devreese B, Nugteren-Roodzant I M, Crielaard W, Boelens R, Kaptein R, van Beeumen J, Hellingwerf K J
机构信息
Department of Microbiology, E. C. Slater Institute, University of Amsterdam, The Netherlands.
出版信息
Biochemistry. 1994 Nov 29;33(47):13959-62. doi: 10.1021/bi00251a001.
A number of Eubacteria contain a photoactive yellow protein which has a photosensory function in negative phototaxis. It has been proposed that the cofactor responsible for the intense yellow color of this protein is retinal [McRee, D. E., et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 6533-6537]. This would make it the first eubacterial rhodopsin. Here we report the chemical structure of this chromophoric group to be p-coumaric acid, which is covalently bound to a unique cysteine in the apoprotein via a thiol ester bond, and thus not retinal. This makes PYP the first example of a protein containing p-coumaric acid, a metabolite previously found only in plants, as a prosthetic group and establishes the photoactive yellow proteins as a new type of photochemically active receptor molecule.
许多真细菌含有一种光活性黄色蛋白，该蛋白在负趋光性中具有光感功能。有人提出，负责该蛋白强烈黄色的辅因子是视黄醛[麦克里，D. E.等人（1989年）《美国国家科学院院刊》86卷，6533 - 6537页]。这将使其成为首个真细菌视紫红质。在此我们报告，这种发色团的化学结构为对香豆酸，它通过硫酯键与脱辅基蛋白中一个独特的半胱氨酸共价结合，因而不是视黄醛。这使得PYP成为首个含有对香豆酸（一种此前仅在植物中发现的代谢产物）作为辅基的蛋白实例，并将光活性黄色蛋白确立为一种新型的光化学活性受体分子。
Zotero 插件