Department of Radiology and Nuclear Medicine, Imaging Division, University Medical Centre Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
Pharm Res. 2010 Oct;27(10):2205-12. doi: 10.1007/s11095-010-0226-3. Epub 2010 Jul 31.
The present study introduces the preparation and in vitro characterization of a nanoparticle device comprising holmium acetylacetonate for radioablation of unresectable solid malignancies.
HoAcAc nanoparticles were prepared by dissolving holmium acetylacetonate in chloroform, followed by emulsification in an aqueous solution of a surfactant and evaporation of the solvent. The diameter, surface morphology, holmium content, and zeta potential were measured, and thermal behavior of the resulting particles was investigated. The stability of the particles was tested in HEPES buffer. The r(2)* relaxivity of protons and mass attenuation coefficient of the nanoparticles were determined. The particle diameter and surface morphology were studied after neutron activation.
Spherical particles with a smooth surface and diameter of 78 ± 10 nm were obtained, and the particles were stable in buffer. Neutron irradiation did not damage the particles, and adequate amounts of activity were produced for nuclear imaging and radioablation of malignancies through intratumoral injections.
The present study demonstrates that HoAcAc nanoparticles were prepared using a solvent evaporation process. The particle diameter can easily be adapted and can be optimized for specific therapeutic applications and tumor types.
本研究介绍了一种包含乙酰丙酮合钬的纳米颗粒装置的制备和体外特性，用于不可切除的实体恶性肿瘤的放射消融。
通过将乙酰丙酮合钬溶解在氯仿中，然后在表面活性剂的水溶液中乳化并蒸发溶剂来制备 HoAcAc 纳米颗粒。测量了直径、表面形态、钬含量和 Zeta 电位，并研究了所得颗粒的热行为。在 HEPES 缓冲液中测试了颗粒的稳定性。测定了质子的 r(2)*弛豫率和纳米颗粒的质量衰减系数。研究了经中子激活后的颗粒直径和表面形态。
得到了具有光滑表面和 78±10nm 直径的球形颗粒，并且颗粒在缓冲液中稳定。中子辐照没有损坏颗粒，并且通过肿瘤内注射可以产生足够的放射性活度用于核成像和恶性肿瘤的放射消融。
本研究表明，使用溶剂蒸发法制备了 HoAcAc 纳米颗粒。可以轻松调整颗粒直径，并针对特定的治疗应用和肿瘤类型进行优化。
