Amur State University, Blagoveschensk, Russia.
J Biophotonics. 2011 Mar;4(3):206-12. doi: 10.1002/jbio.201000046.
Continuous-wave laser micro-beams are generally used as diagnostic tools in laser scanning microscopes or in the case of near-infrared (NIR) micro-beams, as optical traps for cell manipulation and force characterization. Because single beam traps are created with objectives of high numerical aperture, typical trapping intensities and photon flux densities are in the order of 10(6) W/cm(2) and 10(3) cm(-2) s(-1), respectively. The main idea of our theoretical study was to investigate the thermal reaction of RBCs irradiated by laser micro-beam. The study is supported by the fact that many experiments have been carried out with RBCs in laser NIR tweezers. In the present work it has been identified that the laser affects a RBC with a density of absorbed energy at approximately 10(7) J/cm(3), which causes a temperature rise in the cell of about 7-12 °C.
连续波激光微束通常用作激光扫描显微镜中的诊断工具，或者在近红外 (NIR) 微束的情况下，用作光学陷阱以用于细胞操作和力特性描述。由于单光束陷阱是通过高数值孔径的物镜创建的，因此典型的捕获强度和光子通量密度分别为 10(6) W/cm(2) 和 10(3) cm(-2) s(-1)。我们的理论研究的主要思想是研究激光微束辐照 RBC 时的热反应。这项研究得到了在激光近红外镊子中对 RBC 进行了许多实验的支持。在目前的工作中已经确定，激光以大约 10(7) J/cm(3)的吸收能量密度作用于 RBC，这会导致细胞温度升高约 7-12°C。
