Chowdhury Aniket, Waghmare Deepak, Dasgupta Raktim, Majumder Shovan K
Laser Biomedical Applications Section, Raja Ramanna Centre of Advanced Technology, Indore, India.
Department of Physical Sciences, Homi Bhabha National Institute, Mumbai, India.
J Biophotonics. 2018 Aug;11(8):e201700222. doi: 10.1002/jbio.201700222. Epub 2018 May 10.
Rapid membrane damage of optically trapped red blood cells (RBCs) was observed at trapping powers ≥280 mW. An excellent agreement between the estimated laser-induced thermal gradient across trapped cell's membrane and that typically required for membrane electropermeabilization suggests a mechanism involving temperature gradient-induced electropermeabilization of membrane. Also the rapid collapse of the trapped cell due to membrane rupture was seen to cause shock waves in the surroundings permeabilizing nearby untrapped cells. When the experiments were carried out with RBCs collected from type II diabetic patients, a noticeable change in the damage rate compared to normal RBCs was seen suggesting a novel optical diagnosis method for the disease.
在捕获功率≥280 mW时，观察到光镊捕获的红细胞（RBC）出现快速膜损伤。估计的跨捕获细胞膜的激光诱导热梯度与通常膜电穿孔所需的热梯度之间的极佳一致性表明，存在一种涉及温度梯度诱导膜电穿孔的机制。此外，还观察到由于膜破裂导致的捕获细胞快速塌陷，从而在周围环境中产生冲击波，使附近未捕获的细胞通透性增加。当对从II型糖尿病患者采集的红细胞进行实验时，与正常红细胞相比，损伤率出现了明显变化，这表明该疾病存在一种新的光学诊断方法。
