Blázquez-Castro Alfonso
Department of Physics of Materials, Faculty of Sciences, Autonomous University of Madrid, 28049 Madrid, Spain.
Micromachines (Basel). 2019 Jul 31;10(8):507. doi: 10.3390/mi10080507.
For several decades optical tweezers have proven to be an invaluable tool in the study and analysis of myriad biological responses and applications. However, as with every tool, they can have undesirable or damaging effects upon the very sample they are helping to study. In this review the main negative effects of optical tweezers upon biostructures and living systems will be presented. There are three main areas on which the review will focus: linear optical excitation within the tweezers, non-linear photonic effects, and thermal load upon the sampled volume. Additional information is provided on negative mechanical effects of optical traps on biological structures. Strategies to avoid or, at least, minimize these negative effects will be introduced. Finally, all these effects, undesirable for the most, can have positive applications under the right conditions. Some hints in this direction will also be discussed.
几十年来，光镊已被证明是研究和分析无数生物反应及应用的宝贵工具。然而，与每一种工具一样，它们可能会对其所帮助研究的样本产生不良或破坏性影响。在本综述中，将介绍光镊对生物结构和生命系统的主要负面影响。综述将聚焦于三个主要方面：光镊内的线性光激发、非线性光子效应以及采样体积上的热负荷。还提供了关于光阱对生物结构的负面机械效应的额外信息。将介绍避免或至少最小化这些负面影响的策略。最后，所有这些大多不受欢迎的效应在适当条件下可能会有积极的应用。也将讨论在这方面的一些提示。
