Federal University of Pernambuco, Laboratory of Biomedical Optics and Imaging, Avenida da Arquitetura, s/n, Cidade Universitária, Recife, Pernambuco 50740-530, Brazil.
Federal University of Pernambuco, Laboratory of Biomedical Optics and Imaging, Avenida da Arquitetura, s/n, Cidade Universitária, Recife, Pernambuco 50740-530, BrazilbFederal University of Pernambuco, Colégio de Aplicação, Avenida da Arquitetura, s/n, Cid.
J Biomed Opt. 2016 Jul 1;21(7):75012. doi: 10.1117/1.JBO.21.7.075012.
We evaluated the damage caused to optically trapped red blood cells (RBCs) after 1 or 2 min of exposure to near-infrared (NIR) laser beams at 785 or 1064 nm. Damage was quantified by measuring cell elasticity using an automatic, real-time, homemade, optical tweezer system. The measurements, performed on a significant number (hundreds) of cells, revealed an overall deformability decrease up to ∼104% after 2 min of light exposure, under 10 mW optical trapping for the 785-nm wavelength. Wavelength dependence of the optical damage is attributed to the light absorption by hemoglobin. The results provided evidence that RBCs have their biomechanical properties affected by NIR radiation. Our findings establish limits for laser applications with RBCs.
我们评估了在 785nm 或 1064nm 近红外（NIR）激光照射下，红细胞（RBC）在 1 或 2 分钟内受到的损伤。通过使用自动、实时、自制的光镊系统测量细胞弹性来定量损伤。对大量（数百个）细胞进行的测量表明，在 10mW 的光捕获下，785nm 波长的光照射 2 分钟后，细胞整体变形性下降了约 104%。光损伤的波长依赖性归因于血红蛋白对光的吸收。结果表明，NIR 辐射会影响 RBC 的生物力学特性。我们的发现为 RBC 与激光的应用确立了限制。
