OBJECTIVE

To establish the analytical method for the fingerprint of Cortex Phellodendri by NACE-DAD and estimate the quality of Cortex Phellodendri from different habitats and species.

METHODS

Based on the mode of nonaqueous capillary electrophoresis, 40 mmol/L sodium acetate and 40 mmol/L ammonium acetate methanol solution was selected for the buffer (pH 5.8). The separation voltage was 25 kV and detection wavelength was set at 210 nm. Berberine was used as a reference standard, the chromatographic fingerprint was determined. The data were analysed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software to compare the similarity of samples.

RESULTS

NACE-DAD fingerprints with 9 common peaks were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Cortex Phellodendri and its processed products, there were obvious differences in the relative areas of common peaks.

CONCLUSION

The method is reliable, accurate and can be used for quality control of Cortex Phellodendri.