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罗汉果果实中多糖SGPS2的研究

[Studies on the polysaccharide SGPS2 from the fruits of Siraitia grosvenorii].

作者信息

Huang Cui-Ping, Li Jun, Liu Qing-Ye, Lu Xue-Wen, Meng Ai-Ping, Zhu Sheng-Jun

机构信息

Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, School of Environment and Resource of Guangxi Normal University, Guilin 541004, China.

出版信息

Zhong Yao Cai. 2010 Mar;33(3):376-9.

PMID:20681302
Abstract

OBJECTIVE

To study the structure of the polysaccharides constituents in the fruits of Siraitia grosvenorii (Swingle) C. Jeffrey.

METHODS

SGPS2 was purified by DEAE-cellulose and Sephadex G-200 column chromatography, the component and structure of SGPS2 were analyzed on the basis of spectral and chemical studies by HPLC, IR analysis, partial hydrolysis with acid, methylation analysis, GC and 13C-NMR.

RESULTS

The molecular weight of SGPS2 was 650 000. Polysaccharide was composed of L- made up of (1 --> 2, 4) linked rhamnose, (1 --> 4) linked rhamnose residues in main chain; and (1 --> 2) linked rhamnose, (1 --> 3) linked rhamnose in side chains. Terminal residues attached main chain to rhamnose.

CONCLUSION

SGPS2 was composed of rhamnose and curonic acid.

摘要

目的

研究罗汉果(Siraitia grosvenorii (Swingle) C. Jeffrey)果实中多糖成分的结构。

方法

采用DEAE - 纤维素和Sephadex G - 200柱色谱法纯化SGPS2,通过高效液相色谱(HPLC)、红外光谱分析(IR)、酸部分水解、甲基化分析、气相色谱(GC)和13C - 核磁共振(13C - NMR)等光谱和化学研究方法对SGPS2的成分和结构进行分析。

结果

SGPS2的分子量为650 000。多糖由L - 鼠李糖组成,主链由(1→2,4)连接的鼠李糖、(1→4)连接的鼠李糖残基组成;侧链由(1→2)连接的鼠李糖、(1→3)连接的鼠李糖组成。末端残基将主链连接到鼠李糖上。

结论

SGPS2由鼠李糖和糖醛酸组成。

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