Dickson A J, Anderson C E, Langslow D R
Mol Cell Biochem. 1978 Apr 11;19(2):81-92. doi: 10.1007/BF00232598.
The rapid isolation of high yields of parenchymal cells from chicken liver is described. Stringent tests of viability show that the isolated hepatocytes are both structurally and metabolically similar to those in intact liver. During incubation viability decreased and the significance of this change on the interpretation of metabolic experiments is discussed. Lactate was a much more effective gluconeogenic precursor than pyruvate even in the presence of additional reducing equivalents. Hepatocytes isolated from fed chickens produced glucose from glycogen degradation. Glycogenolysis was stimulated by glucagon, dibutyryl cyclic AMP and adrenaline. Half maximal glucagon effects were elicited by physiological concentrations of the hormone. Glucagon and dibutyryl cyclic AMP stimulated glucagon, dibutyryl cyclic AMP and adrenaline their action was not additive to that of adrenaline.
本文描述了从鸡肝脏中快速分离出高产率实质细胞的方法。严格的活力测试表明，分离出的肝细胞在结构和代谢方面均与完整肝脏中的肝细胞相似。在孵育过程中，细胞活力下降，并讨论了这种变化对代谢实验解释的意义。即使在存在额外还原当量的情况下，乳酸也是比丙酮酸更有效的糖异生前体。从喂食后的鸡中分离出的肝细胞通过糖原降解产生葡萄糖。胰高血糖素、二丁酰环磷酸腺苷和肾上腺素可刺激糖原分解。该激素的生理浓度可引发半最大胰高血糖素效应。胰高血糖素和二丁酰环磷酸腺苷刺激胰高血糖素、二丁酰环磷酸腺苷和肾上腺素，它们的作用与肾上腺素的作用并非相加关系。
