Onoagbe I O
Department of Biochemistry, University of Maiduguri, Nigeria.
Exp Cell Res. 1993 Nov;209(1):1-5. doi: 10.1006/excr.1993.1277.
Hepatocytes were isolated from 15-, 16-, 17-, and 18-day-old chick embryos. Glucagon, adrenaline, and dibutyryl cAMP (Bt2cAMP), individually or in combination, activated glycogenolysis in the isolated hepatocytes. The alpha-adrenergic agonist phenylephrine did not increase glycogen breakdown. The action of adrenaline was abolished upon treatment of hepatocytes with a combination of the hormone and propranolol, a beta-adrenergic blocker. The effects of glucagon, adrenaline, and dibutyryl cAMP on glycogenolysis were not additive. Either hormone induced an increase in the concentration of cAMP. The activities of dephosphophosphorylase kinase and phosphorylase a were stimulated by each hormone or Bt2cAMP. It appears likely that glucagon and adrenaline serve as physiological regulators of hepatic glycogen breakdown during embryogenesis in chickens.
从15日、16日、17日和18日龄的鸡胚中分离出肝细胞。胰高血糖素、肾上腺素和二丁酰环磷腺苷（Bt2cAMP）单独或联合使用，均可激活分离出的肝细胞中的糖原分解。α-肾上腺素能激动剂去氧肾上腺素不会增加糖原分解。用激素和β-肾上腺素能阻滞剂普萘洛尔联合处理肝细胞后，肾上腺素的作用消失。胰高血糖素、肾上腺素和二丁酰环磷腺苷对糖原分解的作用并非相加性的。两种激素均可诱导环磷腺苷浓度升高。去磷酸化磷酸化酶激酶和磷酸化酶a的活性受到每种激素或Bt2cAMP的刺激。胰高血糖素和肾上腺素似乎可能是鸡胚胎发育过程中肝脏糖原分解的生理调节因子。
