血清 IL-23 水平升高与人类结直肠癌中 VEGF 的表达相关。

Elevated serum level of IL-23 correlates with expression of VEGF in human colorectal carcinoma.

机构信息

Center for Molecular Medicine, Faculty of Medicine, University of Kragujevac, Kragujevac, Serbia.

出版信息

Arch Med Res. 2010 Apr;41(3):182-9. doi: 10.1016/j.arcmed.2010.02.009. Epub 2010 Mar 19.

DOI:10.1016/j.arcmed.2010.02.009

PMID:20682175

Abstract

BACKGROUND AND AIMS

Interleukin-23 (IL-23) has a role in inflammatory bowel diseases (IBD) as a condition of higher risk of colorectal carcinogenesis. Vascular endothelial growth factor (VEGF) is overexpressed in IBD and colorectal carcinoma. Therefore, we aimed at uncovering the relationship between serum level of IL-23 and expression of VEGF in colorectal cancer (CRC) patients and to establish the relationship between VEGF and p53 and serum levels of IL-23, as well as its possible role in carcinogenesis of colorectal carcinomas.

METHODS

Levels of IL-23 from serum samples of patients with colorectal carcinoma (n = 40) and healthy control samples (n = 37) were examined for IL-23-Ab using an ELISA assay. We also determined the expression of VEGF and p53 by immunohistochemistry in 59 cases of CRC.

RESULTS

We found significantly higher serum levels of IL-23 in patients with CRC compared to control subjects (IL-23; mean 189.46 pg/mL vs. mean 34.77 pg/mL, p = 0.033). We also detected higher serum levels of IL-23 in patients with overexpressed VEGF (p = 0.028). Our results also showed that concomitant expression of VEGF and increased serum levels of IL-23 are in positive correlation with histological grade 2 (p <0.05).

CONCLUSIONS

Our data indicate that serum IL-23 levels are significantly elevated in CRC vs. control patients and are strongly associated with overexpression of VEGF, thus they may play an important role in carcinogenesis of CRC.

摘要

背景与目的

白细胞介素-23（IL-23）在炎症性肠病（IBD）中起作用，是结直肠癌发生的高危因素。血管内皮生长因子（VEGF）在 IBD 和结直肠癌中过度表达。因此，我们旨在揭示结直肠癌（CRC）患者血清中 IL-23 水平与 VEGF 表达之间的关系，并建立 VEGF 与 p53 与血清中 IL-23 水平之间的关系，以及其在结直肠癌发生中的可能作用。

方法

使用 ELISA 法检测 40 例 CRC 患者和 37 例健康对照者血清中 IL-23-Ab 的水平。我们还通过免疫组织化学方法检测了 59 例 CRC 中 VEGF 和 p53 的表达。

结果

与对照组相比，CRC 患者血清中 IL-23 水平显著升高（IL-23；均值 189.46 pg/mL 与均值 34.77 pg/mL，p = 0.033）。我们还检测到 VEGF 过表达的患者血清中 IL-23 水平升高（p = 0.028）。我们的结果还表明，VEGF 表达增加与组织学分级 2 呈正相关（p <0.05）。