Restorative Dentistry, School of Dental Medicine, University at Buffalo, NY 14214, USA.
Biomed Mater. 2010 Aug;5(4):044106. doi: 10.1088/1748-6041/5/4/044106. Epub 2010 Aug 3.
With an electron-beam evaporation process, a calcium phosphate (Ca-P) thin film of approximately 500 nm thick was deposited on sand blasted with large grits and acid etched (SLA) Ti without changing the typical morphology of the SLA surface. Dissolution behavior was investigated by measuring the amount of dissolved phosphate ions with ion chromatography after immersing the SLA Ti sample coated with a Ca-P film in 1 ml de-ionized water maintained at 37 degrees C for different periods of soaking time, and the surface morphology was observed with field emission scanning electron microscopy. The amount of phosphate ions increased quickly right after immersion but began to decrease after 2 days of immersion by redeposition with Ca ions as apatite, and the amount of biomimetic apatite increased with the extended soaking time. The Saos-2 cell was more attached on the coated surface, and the in vivo evaluation was that the Ca-P deposited SLA implant greatly improved the new bone formation ability.
采用电子束蒸发工艺，在喷砂（SLA）钛表面沉积约 500nm 厚的磷酸钙（Ca-P）薄膜，同时保持 SLA 表面的典型形貌不变。将涂覆有 Ca-P 薄膜的 SLA Ti 样品浸入 1ml 去离子水中，在 37°C 下保持不同的浸泡时间，通过离子色谱测量溶解的磷酸盐离子的量来研究溶解行为，并用场发射扫描电子显微镜观察表面形貌。浸泡后，磷酸盐离子的含量迅速增加，但在浸泡 2 天后，由于钙的再沉积而开始减少，形成磷灰石。随着浸泡时间的延长，仿生磷灰石的含量增加。Saos-2 细胞在涂层表面的附着性更好，体内评价表明，沉积 Ca-P 的 SLA 植入物大大提高了新骨形成能力。
