Gubash S M
University of Calgary, Alberta, Canada.
Res Microbiol. 1991 Jan;142(1):87-93. doi: 10.1016/0923-2508(91)90100-o.
A new modification of the egg-yolk agar plate medium, an egg-yolk triple-salt medium, is described. The medium incorporates a tryptic soy agar base, yeast extract, D-glucose CaCl2, MgCl2 and ZnSO4. Only in egg-yolk triple-salt medium did all tested phospholipase-C-producing clostridia form a lecitho-vitellin precipitate after overnight incubation; 95.5% formed a high-density precipitate in the new medium as opposed to 6.1%, 6.6%, 10.6% and 20.2% of the isolates not forming a visible precipitate in liver-veal, Lombard-Dowell, McClung and Toabe modified, and "viande-levure" egg-yolk media, respectively, after the same incubation time.
本文描述了一种蛋黄琼脂平板培养基的新改良方法，即蛋黄三盐培养基。该培养基含有胰蛋白胨大豆琼脂基础培养基、酵母提取物、D - 葡萄糖、氯化钙、氯化镁和硫酸锌。只有在蛋黄三盐培养基中，所有测试的产磷脂酶C梭状芽孢杆菌在过夜培养后都会形成卵磷脂 - 卵黄磷蛋白沉淀；在新培养基中，95.5%的菌株形成了高密度沉淀，而在相同培养时间后，在肝 - 小牛肉、伦巴德 - 道威尔、麦克伦和托阿贝改良的以及“肉 - 酵母”蛋黄培养基中，分别有6.1%、6.6%、10.6%和20.2%的分离株未形成可见沉淀。
