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Appl Environ Microbiol. 1976 May;31(5):784-6. doi: 10.1128/aem.31.5.784-786.1976.
2
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本文引用的文献

1
The biochemistry of the gas gangrene toxins: Estimation of the alpha toxin of Cl. welchii, type A.气性坏疽毒素的生物化学：A型魏氏梭菌α毒素的测定
Biochem J. 1941 Nov;35(10-11):1246-56. doi: 10.1042/bj0351246.
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A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
3
Phospholipid metabolism.磷脂代谢
Annu Rev Biochem. 1972;41(10):129-60. doi: 10.1146/annurev.bi.41.070172.001021.
4
A sensitive and specific plate test for the quantitation of phospholipases.
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用于检测微生物磷脂酶的卵磷脂琼脂。

Lecithin agar for detection of microbial phospholipases.

作者信息

Chrisope G L, Fox C W, Marshall R T

出版信息

Appl Environ Microbiol. 1976 May;31(5):784-6. doi: 10.1128/aem.31.5.784-786.1976.

DOI:10.1128/aem.31.5.784-786.1976
PMID:818960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC291196/
Abstract

Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days earlier than on egg yolk agar. All constituents of the medium can be autoclaved.

摘要

卵磷脂琼脂被研发出来，在其上磷脂酶C会产生浑浊区，而磷脂酶A会产生清亮区。在卵磷脂琼脂上的反应与在蛋黄肉汤中的反应有74%的时间是一致的。在卵磷脂琼脂上，判读更容易，磷脂酶A可被检测到，并且不透明区比在蛋黄琼脂上早1至2天可见。该培养基的所有成分都可进行高压灭菌。