内质网为黄病毒复制复合物的生物发生提供了膜平台。
The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex.
机构信息
Department of Microbiology, La Trobe University, Bundoora, Melbourne 3086, Australia.
出版信息
J Virol. 2010 Oct;84(20):10438-47. doi: 10.1128/JVI.00986-10. Epub 2010 Aug 4.
Abstract
The cytoplasmic replication of positive-sense RNA viruses is associated with a dramatic rearrangement of host cellular membranes. These virus-induced changes result in the induction of vesicular structures that envelop the virus replication complex (RC). In this study, we have extended our previous observations on the intracellular location of West Nile virus strain Kunjin virus (WNV(KUN)) to show that the virus-induced recruitment of host proteins and membrane appears to occur at a pre-Golgi step. To visualize the WNV(KUN) replication complex, we performed three-dimensional (3D) modeling on tomograms from WNV(KUN) replicon-transfected cells. These analyses have provided a 3D representation of the replication complex, revealing the open access of the replication complex with the cytoplasm and the fluidity of the complex to the rough endoplasmic reticulum. In addition, we provide data that indicate that a majority of the viral RNA species housed within the RC is in a double-stranded RNA (dsRNA) form.
摘要
正链 RNA 病毒的细胞质复制与宿主细胞膜的剧烈重排有关。这些病毒诱导的变化导致囊泡结构的诱导，这些囊泡结构包裹着病毒复制复合物（RC）。在这项研究中，我们扩展了之前关于西尼罗河病毒株 Kunjin 病毒（WNV(KUN)）在细胞内位置的观察结果，表明病毒诱导的宿主蛋白和膜的募集似乎发生在高尔基体前步骤。为了可视化 WNV(KUN)复制复合物，我们对来自 WNV(KUN)复制子转染细胞的断层扫描图像进行了三维（3D）建模。这些分析提供了复制复合物的 3D 表示，揭示了复制复合物与细胞质的开放访问和复合物到粗糙内质网的流动性。此外，我们提供的数据表明，RC 内的大多数病毒 RNA 物种都处于双链 RNA（dsRNA）形式。
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