Morin Ryan D, Zhao Yongjun, Prabhu Anna-Liisa, Dhalla Noreen, McDonald Helen, Pandoh Pawan, Tam Angela, Zeng Thomas, Hirst Martin, Marra Marco
BC Cancer Agency, Genome Sciences Centre, Vancouver, BC, Canada.
Methods Mol Biol. 2010;650:173-99. doi: 10.1007/978-1-60761-769-3_14.
MicroRNAs are key regulators of gene expression in diverse biological processes and their importance in embryonic stem cells is indisputable. New 'next-generation' technologies such as Illumina massively parallel sequencing offer vast improvements, in both scale and sensitivity, to microRNA profiling studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for analyzing the resultant sequence data for measuring microRNA abundance.
微小RNA是多种生物学过程中基因表达的关键调节因子，其在胚胎干细胞中的重要性毋庸置疑。诸如Illumina大规模平行测序等新型“下一代”技术，在规模和灵敏度方面都为微小RNA谱分析研究带来了巨大改进。我们描述了一种用于制备Illumina测序小RNA文库的详细程序。我们还对分析所得序列数据以测量微小RNA丰度的方法进行了评论。
