miRNA 谱分析在多发性硬化症中的生物标志物发现：从微阵列到深度测序。
miRNA profiling for biomarker discovery in multiple sclerosis: from microarray to deep sequencing.
机构信息
Neurology Department, The Ohio State University Medical Center, 460 W 12th Ave Room 0705, Columbus, OH 43210, USA.
出版信息
J Neuroimmunol. 2012 Jul 15;248(1-2):32-9. doi: 10.1016/j.jneuroim.2011.10.006. Epub 2011 Nov 9.
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. miRNAs are highly expressed in cells of the immune and nervous system, attesting to their importance in Neuroimmunology. Besides their involvement in modulation of physiological and pathological processes, miRNAs hold high promise as disease biomarkers, therapeutic agents and/or drug targets. Several studies have recently explored the involvement of miRNAs in Multiple Sclerosis (MS) using a variety of miRNA profiling techniques. In this review, we discuss basic miRNA biology and nomenclature, the techniques available for miRNA profiling research and recent miRNA profiling studies in Multiple Sclerosis.
摘要
MicroRNAs (miRNAs) 是小的非编码 RNA，可以在转录后水平调控基因表达。miRNAs 在免疫和神经系统细胞中高度表达，证明了它们在神经免疫学中的重要性。除了参与调节生理和病理过程外，miRNAs 作为疾病生物标志物、治疗剂和/或药物靶点具有很高的应用前景。最近，使用多种 miRNA 分析技术，多项研究探讨了 miRNA 在多发性硬化症（MS）中的作用。在这篇综述中，我们讨论了 miRNA 的基本生物学和命名法、用于 miRNA 分析研究的技术以及多发性硬化症的最新 miRNA 分析研究。
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本文引用的文献
1
Micro-RNA dysregulation in multiple sclerosis favours pro-inflammatory T-cell-mediated autoimmunity.
Brain. 2011 Dec;134(Pt 12):3578-89. doi: 10.1093/brain/awr262. Epub 2011 Nov 15.
2
Deep sequencing of microRNA precursors reveals extensive 3' end modification.
RNA. 2011 Oct;17(10):1795-803. doi: 10.1261/rna.2713611. Epub 2011 Aug 17.
3
Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity.
Genome Res. 2011 Sep;21(9):1450-61. doi: 10.1101/gr.118059.110. Epub 2011 Aug 3.
4
Widespread regulatory activity of vertebrate microRNA* species.
RNA. 2011 Feb;17(2):312-26. doi: 10.1261/rna.2537911. Epub 2010 Dec 22.
5
miRBase: integrating microRNA annotation and deep-sequencing data.
Nucleic Acids Res. 2011 Jan;39(Database issue):D152-7. doi: 10.1093/nar/gkq1027. Epub 2010 Oct 30.
6
In situ detection of microRNAs in paraffin embedded, formalin fixed tissues and the co-localization of their putative targets.
Methods. 2010 Dec;52(4):307-15. doi: 10.1016/j.ymeth.2010.08.009. Epub 2010 Aug 17.
7
MicroRNAs miR-17 and miR-20a inhibit T cell activation genes and are under-expressed in MS whole blood.
PLoS One. 2010 Aug 11;5(8):e12132. doi: 10.1371/journal.pone.0012132.
8
Normalization strategies for microRNA profiling experiments: a 'normal' way to a hidden layer of complexity?
Biotechnol Lett. 2010 Dec;32(12):1777-88. doi: 10.1007/s10529-010-0380-z. Epub 2010 Aug 12.
9
Preparation and analysis of microRNA libraries using the Illumina massively parallel sequencing technology.
Methods Mol Biol. 2010;650:173-99. doi: 10.1007/978-1-60761-769-3_14.
10
Altered miRNA expression in T regulatory cells in course of multiple sclerosis.
J Neuroimmunol. 2010 Sep 14;226(1-2):165-71. doi: 10.1016/j.jneuroim.2010.06.009. Epub 2010 Jul 16.
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