CESA-Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
Ecotoxicol Environ Saf. 2010 Sep;73(6):1255-63. doi: 10.1016/j.ecoenv.2010.07.027. Epub 2010 Aug 4.
The effects of sewage sludge selectively enriched with Cd and Zn, both singly and in combination, on the bacterial, fungal, Alphaproteobacteria and Actinobacteria communities of a soil under arable or grassland management were studied with a PCR-DGGE approach. The effects of Cd and Zn were evaluated after a short time (7 d) when the Cd and Zn solubility were low and the C availability was high, and again after 180 d when the labile sludge C was mineralized and the effects of heavy metals predominated. In the arable soil all treatments induced significant short-term changes in the studied microbial groups, and long-term changes were observed in Actinobacteria and fungal communities. In the grassland soil, all treatments induced significant short-term changes in the studied microbial groups except for Alphaproteobacteria and fungi, and long-term effects on the actinobacteria and fungal communities. It was concluded that incorporation of Cd- and Zn-rich sludge into soils may have both short- and long-term effects on various bacterial phylogenetic groups whereas the metals may be better tolerated by the dominant soil fungi. In this study the impact was greater in arable than in grassland soil.
采用 PCR-DGGE 方法研究了单独及联合添加 Cd 和 Zn 的污水污泥对耕地和草地管理下土壤中细菌、真菌、α变形菌和放线菌群落的影响。在 Cd 和 Zn 溶解度低且 C 供应高的短期（7 d）和可利用污泥 C 矿化且重金属占主导地位的长期（180 d）条件下，评估了 Cd 和 Zn 的影响。在耕地土壤中，所有处理均导致所研究微生物类群的短期显著变化，并且在放线菌和真菌群落中观察到长期变化。在草地土壤中，除了α变形菌和真菌外，所有处理均导致所研究微生物类群的短期显著变化，并且对放线菌和真菌群落产生长期影响。结论是，将富含 Cd 和 Zn 的污泥掺入土壤中可能会对各种细菌系统发育群产生短期和长期影响，而这些金属可能更容易被优势土壤真菌耐受。在本研究中，耕地土壤的影响大于草地土壤。
