Visual Neuroscience, UCL Institute of Ophthalmology, London, United Kingdom.
Invest Ophthalmol Vis Sci. 2010 Dec;51(12):6504-13. doi: 10.1167/iovs.10-5551. Epub 2010 Aug 4.
Involvement of the outer retina is controversial in glaucoma. The aim of this study was to test, first, whether the outer retina is affected in experimental ocular hypertension (OHT) and, second, whether whole retinal thickness can be used as a surrogate marker of glaucomatous change.
OHT was surgically induced in 20 Dark Agouti rats. Animals were imaged using a modified Spectralis OCT (Heidelberg Engineering, Heidelberg, Germany) at baseline and at 3 and 8 weeks after OHT induction. Measurements were recorded for whole and individual retinal layer thickness in four regions-temporal, superior, nasal, and inferior-around the optic nerve head.
Whole retinal thickness in normal eyes was 172.19 ± 5.17 μm, with no significant regional differences. OHT caused a significant reduction in whole retinal thickness and the outer nuclear layer (ONL) at 3 and 8 weeks (P < 0.05), along with the expected thinning of the retinal nerve fiber layer (RNFL). Whole retinal thickness correlated well with RNFL (P = 0.035) and ONL (P ≤ 0.001) changes. Sensitivity of RNFL and ONL to IOP exposure appeared greater at 3 than at 8 weeks. In addition, regional profiles were significantly altered in the ONL and RNFL after OHT induction.
Adaptation of the Spectralis OCT enables tracking of structural damage in experimental rat OHT. Here the authors show evidence of glaucomatous damage in the outer retinal layers of this model with significant regional changes and highlight whole retinal thickness in the rat as a useful surrogate marker of inner and outer retinal changes. The authors believe that the OCT data can provide useful information with regard to clinical management.
在外伤性青光眼的研究中，视网膜外层的变化仍存在争议。本研究的目的是：首先，检测实验性高眼压（OHT）是否会影响视网膜外层；其次，验证全视网膜厚度是否可作为青光眼变化的替代指标。
通过手术诱导 20 只暗褐家鼠发生 OHT。使用海德堡 Spectralis OCT（德国海德堡工程公司）在基线及 OHT 诱导后 3 周和 8 周对动物进行成像。分别在视神经周围的颞侧、上方、鼻侧和下方 4 个区域记录全视网膜和各层视网膜厚度的测量值。
正常眼的全视网膜厚度为 172.19 ± 5.17μm，且无明显的区域性差异。OHT 在 3 周和 8 周时导致全视网膜厚度和外核层（ONL）显著变薄（P<0.05），同时预期的视网膜神经纤维层（RNFL）也变薄。全视网膜厚度与 RNFL（P=0.035）和 ONL（P≤0.001）的变化密切相关。RNFL 和 ONL 对眼压暴露的敏感性在 3 周时比 8 周时更大。此外，在诱导 OHT 后，ONL 和 RNFL 的区域性形态发生显著改变。
Spectralis OCT 的适应性可用于追踪实验性大鼠 OHT 中的结构损伤。本研究作者证明了这种模型的外层视网膜存在青光眼损伤，并具有明显的区域性变化，同时还强调了大鼠的全视网膜厚度可作为内层和外层视网膜变化的有用替代指标。作者认为 OCT 数据可为临床管理提供有用的信息。
