Krawczyk-Wołoszyn Karolina, Roczkowski Damian, Reich Adam, Żychowska Magdalena
Doctoral School, University of Rzeszow, 35-959 Rzeszów, Poland.
Department of Dermatology, Institute of Medical Sciences, Medical College of Rzeszow University, 35-959 Rzeszów, Poland.
Biomedicines. 2024 Sep 3;12(9):2012. doi: 10.3390/biomedicines12092012.
Penetrating deep into the cells of the human body in real time has become increasingly possible with the implementation of modern technologies in medicine. Atomic force microscopy (AFM) enables the effective live imaging of cellular and molecular structures of biological samples (such as cells surfaces, components of biological membranes, cell nuclei, actin networks, proteins, and DNA) and provides three-dimensional surface visualization (in X-, Y-, and Z-planes). Furthermore, the AFM technique enables the study of the mechanical, electrical, and magnetic properties of cells and cell organelles and the measurements of interaction forces between biomolecules. The technique has found wide application in cancer research. With the use of AFM, it is not only possible to differentiate between healthy and cancerous cells, but also to distinguish between the stages of cancerous conditions. For many years, AFM has been an important tool for the study of neurodegenerative diseases associated with the deposition of peptide amyloid plaques. In recent years, a significant amount of research has been conducted on the application of AFM in the evaluation of connective tissue cell mechanics. This review aims to provide the spectrum of the most important applications of the AFM technique in medicine to date.
随着现代技术在医学中的应用，实时深入人体细胞内部变得越来越可行。原子力显微镜（AFM）能够对生物样本的细胞和分子结构（如细胞表面、生物膜成分、细胞核、肌动蛋白网络、蛋白质和DNA）进行有效的实时成像，并提供三维表面可视化（在X、Y和Z平面）。此外，AFM技术还能够研究细胞和细胞器的机械、电学和磁学性质，以及测量生物分子之间的相互作用力。该技术在癌症研究中得到了广泛应用。使用AFM，不仅可以区分健康细胞和癌细胞，还可以区分癌症病情的阶段。多年来，AFM一直是研究与肽淀粉样斑块沉积相关的神经退行性疾病的重要工具。近年来，关于AFM在评估结缔组织细胞力学方面的应用已经开展了大量研究。本综述旨在介绍迄今为止AFM技术在医学中最重要的应用范围。
