Brokaw Ann, Cobb Brian A
Department of Science, Rocky River High School, Rocky River, OH.
Biochem Mol Biol Educ. 2009 Jul;37(4):243-248. doi: 10.1002/bmb.20308.
Immunology is gaining prominence both in the media as well as on the Advanced Placement (AP) exam in Biology. One of the challenges of teaching modern biological topics such as immunology and biochemistry in the high-school setting is the increased reliance on expensive technology in the research world. To begin to bridge this widening gap, we devised an experiment using a novel macroscale enzyme-linked immunosorbent assay that is suitable for AP-level high-school biology classrooms as well as entry-level collegiate laboratories. This novel method does not require a plate reader for data analysis, but instead relies on more common and inexpensive equipment such as a clinical test tube centrifuge and a simple test tube spectrophotometer. The experimental plan focuses on students measuring antibody concentrations in "unknown" samples and includes the collection and analysis of a standard curve using reagents prepared by the instructor. Students will be introduced to enzyme action, quantitative laboratory technique, antibodies, and the immune system, with the overall goal being to explore and highlight the inherent connections within the fields of biochemistry and immunology.
免疫学在媒体以及生物学大学预修课程（AP）考试中都越来越受关注。在高中阶段教授诸如免疫学和生物化学等现代生物学主题面临的挑战之一是，研究领域对昂贵技术的依赖日益增加。为了开始弥合这一不断扩大的差距，我们设计了一项实验，使用一种新型的宏观酶联免疫吸附测定法，该方法适用于AP水平的高中生物课堂以及入门级大学实验室。这种新方法不需要酶标仪进行数据分析，而是依赖于更常见且便宜的设备，如临床试管离心机和简单的试管分光光度计。实验计划侧重于让学生测量“未知”样品中的抗体浓度，并包括使用教师准备的试剂收集和分析标准曲线。学生将学习酶的作用、定量实验室技术、抗体和免疫系统，总体目标是探索并突出生物化学和免疫学领域内的内在联系。
