创建一个可靠且经济高效的酶联免疫吸附测定（ELISA）模拟。
Creating a Reliable, Cost-Effective ELISA Simulation.
作者信息
Olson Julie, Amolins Michael, Vitiello Peter
机构信息
Science Teacher at Mitchell High School, 920 N. Capital Street, Mitchell, SD 57301.
Science Teacher at Harrisburg High School, PO Box 339, Harrisburg, SD 57032; michael.
出版信息
Am Biol Teach. 2017 Apr;79(4):301-304. doi: 10.1525/abt.2017.79.4.301.
The enzyme-linked immunosorbent assay (ELISA) is a fundamental laboratory technique with direct applications across scientific research and clinical diagnostics as well as everyday life. Unfortunately, many challenges exist that inhibit both its introduction and implementation in the high school biology classroom. We present a reliable yet inexpensive way of effectively simulating this assay, allowing student exposure to several advanced topics, including immunodetection, clinical diagnostics, and qualitative and quantitative colorimetric analysis.
酶联免疫吸附测定（ELISA）是一项基础实验室技术，直接应用于科学研究、临床诊断以及日常生活。不幸的是，存在许多挑战阻碍其在高中生物课堂中的引入和实施。我们提出了一种可靠且经济的方法来有效模拟这种测定，使学生能够接触到几个高级主题，包括免疫检测、临床诊断以及定性和定量比色分析。
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