Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, 3471 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA.
J Orthop Res. 2011 Feb;29(2):270-4. doi: 10.1002/jor.21214.
This study was performed to analyze the role of hemorrhage-induced hypotension in the induction of systemic inflammation and remote organ dysfunction. Male C57/BL6 mice (6- to 10-week old and 20-30 g) were used. Animals were either subjected to pseudo-fracture [PF; standardized soft-tissue injury and injection of crushed bone, PF group: n = 9], or PF combined with hemorrhagic shock (HS + PF group: n = 6). Endpoint was 6 h. Systemic inflammation was assessed by IL-6 and IL-10 levels. Myeloperoxidase (MPO) and NF-κB activity in the lung and liver tissue were obtained to assess remote organ damage. The increases of systemic cytokines are similar for animals subjected to PF and PF + HS (IL-6: 189 pg/ml ± 32.5 vs. 160 pg/ml ± 5.3; IL-10: 60.3 pg/ml ± 15.8 vs. 88 pg/ml ± 32.4). Furthermore, the features (ALT; NF-κB) of liver injury are equally elevated in mice subjected to PF (76.9 U/L ± 4.5) and HS + PF (80 U/L ± 5.5). Lung injury, addressed by MPO activity was more severe in group HS + PF (2.95 ng/ml ± 0.32) than in group PF (1.21 ng/ml ± 0.2). Both PF and additional HS cause a systemic inflammatory response. In addition, hemorrhage seems to be associated with remote affects on the lung.
这项研究旨在分析出血性低血压在全身炎症和远处器官功能障碍诱导中的作用。雄性 C57/BL6 小鼠（6-10 周龄，20-30g）用于研究。动物分为假骨折组（PF；标准化软组织损伤和粉碎骨注射，PF 组：n=9）或 PF 合并失血性休克组（HS+PF 组：n=6）。终点为 6 小时。通过白细胞介素 6（IL-6）和白细胞介素 10（IL-10）水平评估全身炎症。获得肺和肝组织中的髓过氧化物酶（MPO）和 NF-κB 活性以评估远处器官损伤。PF 和 PF+HS 组动物的全身细胞因子增加相似（IL-6：189pg/ml±32.5 与 160pg/ml±5.3；IL-10：60.3pg/ml±15.8 与 88pg/ml±32.4）。此外，PF 组（76.9U/L±4.5）和 HS+PF 组（80U/L±5.5）的肝脏损伤特征（ALT；NF-κB）同样升高。通过 MPO 活性评估的肺损伤在 HS+PF 组（2.95ng/ml±0.32）比 PF 组（1.21ng/ml±0.2）更严重。PF 和附加的 HS 都会引起全身炎症反应。此外，出血似乎与肺部的远处影响有关。
