INEB-Instituto de Engenharia Biomedica, Rua do Campo Alegre No. 823, 4150-180 Porto, Portugal.
Biomacromolecules. 2010 Aug 9;11(8):1956-64. doi: 10.1021/bm100264a.
In this work, human mesenchymal stem cells (hMSC) immobilized in RGD-coupled alginate microspheres, with a binary composition of high and low molecular weight alginate, were investigated. Cells immobilized within RGD-alginate microspheres (during 21 days) showed metabolic activity, with an overall viability higher than 90%, short cell extensions, and, when induced, they were able to differentiate into the osteogenic lineage. In osteogenic conditions (comparing to basal conditions), immobilized cells presented alkaline phosphatase (ALP) activity and an upregulation of ALP, collagen type I, and Runx 2 expression. Moreover, mineralization was also detected in immobilized cells under osteogenic stimulus. In addition, it was demonstrated for the first time that MSCs immobilized in this 3D matrix were able to enhance the ability of neighboring endothelial cells to form tubelike structures. Overall, these findings represent a step forward in the development of injectable stem cell carriers for bone tissue engineering.
在这项工作中，研究了固定在 RGD 偶联海藻酸钠微球中的人骨髓间充质干细胞（hMSC），该微球由高分子量和低分子量海藻酸钠的二元组成。在 RGD-海藻酸钠微球中固定的细胞（在 21 天内）表现出代谢活性，整体存活率高于 90%，细胞短延伸，并且在诱导时能够分化为成骨谱系。在成骨条件下（与基础条件相比），固定化细胞具有碱性磷酸酶（ALP）活性，并上调了 ALP、I 型胶原和 Runx2 的表达。此外，在成骨刺激下还检测到固定化细胞的矿化。此外，首次证明固定在这种 3D 基质中的 MSC 能够增强相邻内皮细胞形成管状结构的能力。总的来说，这些发现代表了在开发用于骨组织工程的可注射干细胞载体方面的一个进步。
