Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, 560-8531, Japan.
Biomacromolecules. 2010 Aug 9;11(8):2179-83. doi: 10.1021/bm100623k.
Hematin, an iron-containing porphyrin used in the management of porphyria attacks, was evaluated as an alternative catalyst to horseradish peroxidase (HRP) for in situ gelation of polymers with phenolic hydroxyl (Ph) moieties in vivo. An aqueous solution of gelatin derivative with Ph moieties was gellable in the presence of both hematin and H2O2. A total of 98.6% adhesion of L929 fibroblast cells 4 h after seeding and their similar morphology to those on substrate coated with unmodified gelatin indicated no obvious substrate cytotoxicity. High cytocompatibility of the gelation process under conditions inducing gelation within 20 s was demonstrated by 95.0% viability of enclosed cells in vitro. Furthermore, no adverse effects of hematin were found compared with HRP by histological observation of cutaneous tissue surrounding in situ formed gels. The versatility of hematin for gelation of a variety of polymers possessing Ph groups was demonstrated by the gelation of a carboxymethyl cellulose derivative.
血红素是一种含铁的卟啉，用于管理卟啉病发作，它被评估为辣根过氧化物酶 (HRP) 的替代催化剂，用于体内具有酚羟基 (Ph) 部分的聚合物的原位凝胶化。在血红素和 H2O2 的存在下，具有 Ph 部分的明胶衍生物的水溶液是可凝胶化的。在接种后 4 小时，L929 成纤维细胞的粘附率达到 98.6%，并且它们的形态与涂有未修饰明胶的基质上的细胞相似，表明没有明显的基质细胞毒性。在 20 秒内诱导凝胶形成的条件下，凝胶化过程具有高细胞相容性，体外封闭细胞的活力达到 95.0%。此外，与 HRP 相比，通过对原位形成的凝胶周围皮肤组织的组织学观察，未发现血红素的不良影响。血红素对具有 Ph 基团的各种聚合物的凝胶化的多功能性通过羧甲基纤维素衍生物的凝胶化得到证明。
