Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Soft Matter. 2019 Mar 6;15(10):2163-2169. doi: 10.1039/c8sm01839a.
In the present work, three kinds of reducing sugars: glucose, galactose, and mannose, are applied to horseradish peroxidase (HRP)-catalyzed hydrogelation of an aqueous solution containing natural polymers modified with phenolic hydroxyl moieties. In this system, HRP consumes hydrogen peroxide that was generated from the oxidation of thiol groups in HRP in the presence of reducing sugars. Herein, we highlight the versatility of applicable sugar types and the controllable hydrogel properties. The mechanical properties and microstructures of the resultant hydrogels can be well controlled by varying the concentration and the reducing power of sugars. Moreover, reducing sugar-independent cytocompatibility of the hydrogels was confirmed by the growth of cells on them. The wide selection of sugar types provides a better understanding of the reaction mechanism and enables the characterization of hydrogels with well-controlled properties.
在本工作中，三种还原糖（葡萄糖、半乳糖和甘露糖）被应用于辣根过氧化物酶（HRP）催化的含有经酚羟基修饰的天然聚合物的水溶液的水凝胶化反应。在该体系中，HRP 消耗了过氧化氢，而过氧化氢是由存在还原糖时 HRP 中巯基基团的氧化产生的。在此，我们强调了适用糖类型的多功能性和可控的水凝胶性质。通过改变糖的浓度和还原能力，可以很好地控制所得水凝胶的机械性能和微观结构。此外，水凝胶的还原糖非依赖性细胞相容性通过细胞在其上的生长得到了证实。糖类型的广泛选择提供了对反应机制的更好理解，并能够对具有良好控制性能的水凝胶进行表征。
