荧光核昔作为天然蛋白质中色氨酸的 FRET 受体。
Fluorescent ribonucleoside as a FRET acceptor for tryptophan in native proteins.
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA.
出版信息
J Am Chem Soc. 2010 Sep 1;132(34):11896-7. doi: 10.1021/ja105244t.
Abstract
A new fluorescent ribonucleoside analogue, containing 5-aminoquinazoline-2,4(1H,3H)-dione, acts as a Forster resonance energy transfer acceptor for tryptophan (R(0) = 22 A) and displays visible emission (440 nm). As tryptophan is frequently found at or near the recognition domains of RNA binding proteins, this FRET pair facilitates the study of RNA binding to native proteins and peptides, which is demonstrated here for the HIV-1 Rev association with the Rev Response Element (RRE).
摘要
一种新型的荧光核苷类似物，含有 5-氨基喹唑啉-2,4(1H,3H)-二酮，可作为色氨酸的福斯特共振能量转移受体（R(0) = 22 A），并显示可见发射（440nm）。由于色氨酸经常出现在 RNA 结合蛋白的识别域或附近，因此这种 FRET 对促进了对天然蛋白质和肽与 RNA 结合的研究，本文展示了 HIV-1 Rev 与 Rev 反应元件（RRE）的结合。
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