Department of Pharmacology, M.V.P.S. College of Pharmacy, Shivaji Nagar, Nashik, India.
Pharm Biol. 2010 Sep;48(9):1038-46. doi: 10.3109/13880200903473741.
Restraint stress is a well-known method to induce chronic stress which leads to alterations in various behavioral and biochemical parameters.
The present work was designed to study anti-stress effects of Morus alba in chronic restraint stress (RS)-induced perturbations in behavioral, biochemical and brain oxidative stress status.
The stress was produced by restraining the animals inside an adjustable cylindrical plastic tube for 3 h once daily for ten consecutive days. The ethyl acetate soluble fraction of Morus alba (EASF) 25, 50, 100 mg/kg and diazepam (1 mg/kg) per day was administered 60 min prior to the stress procedure. The behavioral and biochemical parameters such as open field, cognitive dysfunction; leucocytes count; blood glucose and corticosteroid levels were determined. On day 10, the rats were sacrificed and biochemical assessment of superoxide dismutase (SOD), lipid peroxidation (LPO), catalase (CAT), and glutathione reductase (GSH) in whole rat brain were performed.
Chronic restraint stress produced cognitive dysfunction, altered behavioral parameters, increased leucocytes count, SOD, LPO, glucose and corticosterone levels, with concomitant decrease in CAT and GSH activities. Gastric ulceration, adrenal gland and spleen weights were also used as the stress indices. All these RS induced perturbations were attenuated by EASF of Morus alba.
The results of the study suggest that in addition to its classically established pharmacological activities, the plant also has immense potential as an anti-stress agent of great therapeutic relevance.
This study indicates the beneficial role of Morus alba for the treatment of oxidative stress-induced disorders.
束缚应激是一种诱导慢性应激的常用方法，可导致各种行为和生化参数的改变。
本研究旨在研究桑白皮（Morus alba）对慢性束缚应激（RS）诱导的行为、生化和大脑氧化应激状态改变的抗应激作用。
通过将动物每天一次限制在可调节的圆柱形塑料管内 3 小时来产生应激，连续 10 天。每天在应激程序前 60 分钟给予桑白皮的乙酸乙酯可溶部分（EASF）25、50、100mg/kg 和地西泮（1mg/kg）。测定行为和生化参数，如旷场、认知功能障碍；白细胞计数；血糖和皮质酮水平。在第 10 天，处死大鼠，对整个大鼠大脑中的超氧化物歧化酶（SOD）、脂质过氧化（LPO）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GSH）进行生化评估。
慢性束缚应激导致认知功能障碍、行为参数改变、白细胞计数增加、SOD、LPO、葡萄糖和皮质酮水平升高，同时 CAT 和 GSH 活性降低。胃溃疡、肾上腺和脾脏重量也被用作应激指标。桑白皮的 EASF 减轻了所有这些 RS 诱导的改变。
研究结果表明，除了其经典的药理学活性外，该植物还具有作为具有重要治疗相关性的抗应激剂的巨大潜力。
这项研究表明桑白皮对治疗氧化应激诱导的疾病具有有益作用。
