利用丙酸作为前体在重组谷氨酸棒杆菌中生产聚（3-羟基丁酸-co-3-羟基戊酸）。
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Corynebacterium glutamicum using propionate as a precursor.
机构信息
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.
出版信息
J Biotechnol. 2011 Apr 10;152(4):144-6. doi: 10.1016/j.jbiotec.2010.07.031. Epub 2010 Aug 6.
Lipopolysaccharides free P[3-hydroxybutyrate (3HB)-co-3-hydroxyvalerate (3HV)] production was achieved using recombinant Corynebacterium glutamicum harboring polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha. Cells grown on glucose with feeding of propionate as a precursor of 3HV unit accumulated 8-47wt% of P(3HB-co-3HV). The 3HV fraction in the copolymer was varied from 0 to 28mol% depending on the propionate concentrations.
利用来源于恶臭假单胞菌的聚羟基烷酸（PHA）生物合成基因的重组谷氨酸棒杆菌，实现了无脂多糖的 P[3-羟基丁酸（3HB）-共-3-羟基戊酸（3HV）]的生产。在葡萄糖上生长的细胞通过丙酸作为 3HV 单元的前体进行补料，积累了 8-47wt%的 P（3HB-共-3HV）。共聚物中的 3HV 比例根据丙酸浓度从 0 变化到 28mol%。
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