生产阶段的磷酸盐供给策略可提高真养产碱杆菌对聚（3-羟基丁酸酯-co-3-羟基戊酸酯）的储存量。

Phosphate feeding strategy during production phase improves poly(3-hydroxybutyrate-co-3-hydroxyvalerate) storage by Ralstonia eutropha.

作者信息

Squio C R, Marangoni C, De Vecchi C S, Aragão G M F

机构信息

Chemical and Food Engineering Department, Technological Center, Federal University of Santa Catarina, C.P. 476, 88040-900, Florianópolis, Brazil.

出版信息

Appl Microbiol Biotechnol. 2003 May;61(3):257-60. doi: 10.1007/s00253-003-1258-y. Epub 2003 Mar 5.

DOI:10.1007/s00253-003-1258-y

PMID:12698285

Abstract

The effect of a phosphate feeding strategy and the optimal rate of biomass production ( r(x)) during the production phase of P(3HB-co-3HV) in a 6-l fermentor were determined in cultures of Ralstonia eutropha with the goal of enhancing polymer productivity. Rates of biomass production ( r(x)) between 0.00 and 0.20 gx r l(-1) h(-1) were monitored during the production phase. When a low rate of cell growth was maintained ( r(x) of 0.02 gx r l(-1) h(-1)), polymer production improved, resulting in a final cell mass, P(3HB-co-3HV) mass, and P(3HB-co-3HV) content of 98.2 g, 62.0 g and 63.1 wt%, respectively, after 27.3 h. The maximum polymer productivity obtained during the production phase was 1.36 g l(-1 )h(-1).

摘要

在6升发酵罐中，以提高聚合物生产力为目标，在真养产碱杆菌培养物中确定了磷酸盐进料策略以及聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（P(3HB-co-3HV)）生产阶段生物量生产的最佳速率（r(x)）。在生产阶段监测了生物量生产速率（r(x)）在0.00至0.20 gx r l(-1) h(-1)之间的情况。当维持较低的细胞生长速率（r(x)为0.02 gx r l(-1) h(-1)）时，聚合物产量提高，在27.3小时后，最终细胞质量、P(3HB-co-3HV)质量和P(3HB-co-3HV)含量分别为98.2克、62.0克和63.1重量%。生产阶段获得的最大聚合物生产力为1.36 g l(-1 )h(-1)。