Laboratorio de Endocrinología y Tumores Hormonodependientes, Ciudad Universitaria, Paraje El Pozo, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CP 3000 Santa Fe, Argentina.
Reprod Toxicol. 2010 Dec;30(4):550-7. doi: 10.1016/j.reprotox.2010.07.008. Epub 2010 Aug 6.
We evaluated whether exposure to bisphenol A (BPA) disrupts neonatal follicle development in rats. From postnatal day 1 (PND1) to PND7, pups received corn oil (control), diethylstilbestrol (DES20: 20 μg/kg-d, DES0.2: 0.2 μg/kg-d), or BPA (BPA20: 20mg/kg-d, BPA0.05: 0.05 mg/kg-d). We examined follicular dynamics, multioocyte follicles (MOFs) incidence, proliferation and apoptosis rates, expression of steroid receptors (ERα, ERβ, PR, AR) and cyclin-dependent kinase inhibitor 1B (p27) in PND8 ovaries. DES20, DES0.2 and BPA20-ovaries showed fewer primordial follicles and increased growing follicles. DES20-ovaries exhibited increased incidence of MOFs. Oocyte survival, AR, PR and apoptosis were not changed. Primordial and recruited follicles from BPA20-ovaries showed higher p27, whereas ERβ and proliferation were both increased in recruited follicles. ERα positive primary follicles increased in BPA 20-ovaries. Results show that BPA reduces the primordial follicle pool by stimulating the neonatal initial recruitment, associated with an increased proliferation rate likely mediated by an estrogenic pathway.
我们评估了双酚 A（BPA）暴露是否会破坏大鼠新生卵泡的发育。从出生后第 1 天（PND1）到第 7 天（PND7），幼鼠接受玉米油（对照）、己烯雌酚（DES20：20μg/kg-d、DES0.2：0.2μg/kg-d）或 BPA（BPA20：20mg/kg-d、BPA0.05：0.05mg/kg-d）。我们检查了 PND8 卵巢中的卵泡动力学、多卵母细胞卵泡（MOFs）发生率、增殖和凋亡率、类固醇受体（ERα、ERβ、PR、AR）和细胞周期蛋白依赖性激酶抑制剂 1B（p27）的表达。DES20、DES0.2 和 BPA20 卵巢中的原始卵泡减少，生长卵泡增加。DES20 卵巢中的 MOFs 发生率增加。卵母细胞存活、AR、PR 和凋亡没有变化。BPA20 卵巢中的原始和募集卵泡表现出更高的 p27，而募集卵泡中的 ERβ 和增殖都增加。BPA20 卵巢中的 ERα 阳性初级卵泡增加。结果表明，BPA 通过刺激新生初始募集来减少原始卵泡库，这与增殖率增加有关，可能是通过雌激素途径介导的。
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