Harada M, Akashi K, Shibuya T, Takamatsu Y, Niho Y
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ann N Y Acad Sci. 1991;628:156-64. doi: 10.1111/j.1749-6632.1991.tb17235.x.
We studied the effects of interleukin-4 (IL-4) on the spontaneous proliferation of chronic myelomonocytic leukemia (CMMoL) cells in vitro to clarify whether IL-4 can act as a negative regulator of hematopoiesis. The results clearly show that IL-4 suppressed spontaneous DNA synthesis and colony formation by CMMoL cells in vitro. Colony formation by CMMoL cells was substantially suppressed by adding either an anti-IL-6 or anti-GM-CSF antibody to the colony assay system. This suppression was abrogated by the treatment of IL-4 with an anti-IL-4 antibody. Furthermore, the production of IL-6 or GM-CSF was markedly inhibited by adding IL-4 to the culture system. These observations indicate that IL-4 can act as a negative regulator for the autocrine growth of CMMoL cells by inhibiting their production of IL-6 and/or GM-CSF.
我们研究了白细胞介素-4（IL-4）对慢性粒单核细胞白血病（CMMoL）细胞体外自发增殖的影响，以阐明IL-4是否可作为造血的负调节因子。结果清楚地表明，IL-4在体外抑制了CMMoL细胞的自发DNA合成和集落形成。在集落测定系统中加入抗IL-6或抗GM-CSF抗体，可显著抑制CMMoL细胞的集落形成。用抗IL-4抗体处理IL-4可消除这种抑制作用。此外，向培养系统中加入IL-4可显著抑制IL-6或GM-CSF的产生。这些观察结果表明，IL-4可通过抑制CMMoL细胞产生IL-6和/或GM-CSF，作为其自分泌生长的负调节因子。
