Yanagisawa K, Kishimoto K, Horiuchi T, Takada K, Yasukawa M, Fujita S
First Department of Internal Medicine, School of Medicine, Ehime University, Japan.
Acta Haematol. 1994;92(2):104-8. doi: 10.1159/000204191.
We examined the effects of interleukin-4 (IL-4) on the growth of hematopoietic precursors from a patient with chronic myelomonocytic leukemia (CMMoL). In the chronic phase of the disease, IL-4 inhibited spontaneous colony formation by CMMoL cells in semi-solid culture. However, in the acute phase, IL-4 promoted colony formation. These effects of IL-4 were abolished completely by the addition of anti-IL-4 neutralizing antibodies. The spontaneous colony formation by CMMoL cells in both the chronic and acute phases was inhibited by the addition of anti-IL-6 antibodies. On the other hand, neither anti-IL-6, anti-granulocyte macrophage colony-stimulating factor (GM-CSF), anti-IL-1 beta nor anti-tumor necrosis factor alpha (TNF alpha) antibodies inhibited IL-4-induced colony formation by CMMoL cells in the acute phase. IL-4 suppressed the production of IL-6 by CMMoL cells in both the chronic and acute phases. These results suggest that in the present patient, IL-4 suppressed the IL-6-mediated autocrine growth of CMMoL cells in both the chronic and acute phases, but directly stimulated their growth in the acute phase.
我们研究了白细胞介素-4（IL-4）对一名慢性粒单核细胞白血病（CMMoL）患者造血前体细胞生长的影响。在疾病的慢性期，IL-4抑制了CMMoL细胞在半固体培养中的自发集落形成。然而，在急性期，IL-4促进了集落形成。通过添加抗IL-4中和抗体，IL-4的这些作用被完全消除。添加抗IL-6抗体可抑制CMMoL细胞在慢性期和急性期的自发集落形成。另一方面，抗IL-6、抗粒细胞巨噬细胞集落刺激因子（GM-CSF）、抗IL-1β或抗肿瘤坏死因子α（TNFα）抗体均未抑制CMMoL细胞在急性期由IL-4诱导的集落形成。IL-4在慢性期和急性期均抑制了CMMoL细胞IL-6的产生。这些结果表明，在该患者中，IL-4在慢性期和急性期均抑制了CMMoL细胞IL-6介导的自分泌生长，但在急性期直接刺激了它们的生长。
