Gharbi Amina, Oudadesse Hassane, El Feki Hafedh, Cheikhrouhou-Koubaa Wissem, Chatzistavrou Xanthippi, V Rau Julietta, Heinämäki Jyrki, Antoniac Iulian, Ashammakhi Nureddin, Derbel Nabil
CEM Lab, National Engineering School of Sfax, Sfax University, Sfax 3018, Tunisia.
LT2S Lab, Digital Research Centre of Sfax, Technopole of Sfax, P.O. Box 275, Sfax 3000, Tunisia.
J Funct Biomater. 2023 Jul 11;14(7):364. doi: 10.3390/jfb14070364.
Derived Hench bioactive glass (BaG) containing boron (B) is explored in this work as it plays an important role in bone development and regeneration. B was also found to enhance BaG dissociation. However, it is only possible to incorporate a limited amount of B. To increase the amount of B in BaG, bioactive borosilicate glasses (BaG-B) were fabricated based on the use of the solution-gelation process (sol-gel). In this work, a high B content (20 wt.%) in BaG, respecting the conditions of bioactivity and biodegradability required by Hench, was achieved for the first time. The capability of BaG-B to form an apatite phase was assessed in vitro by immersion in simulated body fluid (SBF). Then, the chemical structure and the morphological changes in the fabricated BaG-B (x = 0, 5, 10 and 20) were studied. The formation of hydroxyapatite (HAp) layer was observed with X-ray diffraction (XRD) and infrared (IR) spectroscopy. The presence of HAp layer was confirmed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Enhanced bioactivity and chemical stability of BaG-B were evaluated with an ion exchange study based on Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) and energy dispersive spectroscopy (EDS). Results indicate that by increasing the concentration of B in BaG-Bx, the crystallization rate and the quality of the newly formed HAp layer on BaG-B surfaces can be improved. The presence of B also leads to enhanced degradation of BaGs in SBF. Accordingly, BAG-B can be used for bone regeneration, especially in children, because of its faster degradation as compared to B-free glass.
在本研究中，我们探索了含硼（B）的衍生亨奇生物活性玻璃（BaG），因为硼在骨骼发育和再生中起着重要作用。研究还发现硼能增强BaG的分解。然而，只能掺入有限量的硼。为了增加BaG中的硼含量，基于溶液凝胶法（溶胶 - 凝胶）制备了生物活性硼硅酸盐玻璃（BaG - B）。在本研究中，首次在符合亨奇所要求的生物活性和生物降解性条件下，在BaG中实现了高硼含量（20 wt.%）。通过将BaG - B浸泡在模拟体液（SBF）中，体外评估了其形成磷灰石相的能力。然后，研究了所制备的BaG - B（x = 0、5、10和20）的化学结构和形态变化。通过X射线衍射（XRD）和红外（IR）光谱观察到了羟基磷灰石（HAp）层的形成。使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）确认了HAp层的存在。基于电感耦合等离子体发射光谱法（ICP - OES）和能量色散光谱法（EDS）的离子交换研究评估了BaG - B增强的生物活性和化学稳定性。结果表明，通过增加BaG - Bx中的硼浓度，可以提高BaG - B表面上新形成的HAp层的结晶速率和质量。硼的存在还导致BaG在SBF中的降解增强。因此，由于与无硼玻璃相比，BaG - B降解更快，它可用于骨再生，尤其是在儿童中。
