Department of Biomedical Engineering, Chung Yuan Christian University, Chung Li, Taiwan, Republic of China.
J Biomed Mater Res A. 2010 Sep 15;94(4):1133-40. doi: 10.1002/jbm.a.32792.
Bamboo charcoal beads (BCBs) were formed by coprecipitating bamboo charcoal particles with chitosan in alkaline solution. The amount of chitosan in the BCBs and their surface properties were measured. When 13-52 mg BCBs were exposed to RAW 264.7 macrophages, the amount of nitric oxide released and the cell viability were close to those of the blank. The amount of cytokine IL-6 secreted by macrophages did not depend on the dose of BCBs but macrophages secreted more TNF-alpha in response to higher doses of BCBs. However, the cytokine levels were relatively low, suggesting the favorable biocompatibility of BCBs. In adsorption experiments, BCBs adsorbed and released bovine serum albumin at particular concentrations, whereas BCBs adsorbed L-phenylalanine without a sign of release. This difference is attributed to the hydrophilicity and the pore size of the BCBs. Finally, the potential of BCBs as biocompatible adsorbents in blood detoxification is considered.
竹炭珠（BCBs）是通过在碱性溶液中与壳聚糖共沉淀竹炭颗粒形成的。测量了 BCBs 中的壳聚糖含量及其表面性质。当 13-52mg 的 BCBs 暴露于 RAW 264.7 巨噬细胞时，释放的一氧化氮量和细胞活力与空白组相近。巨噬细胞分泌的细胞因子 IL-6 的量不取决于 BCBs 的剂量，但巨噬细胞对更高剂量的 BCBs 分泌更多的 TNF-α。然而，细胞因子水平相对较低，表明 BCBs 具有良好的生物相容性。在吸附实验中，BCBs 在特定浓度下吸附和释放牛血清白蛋白，而 BCBs 吸附 L-苯丙氨酸而没有释放的迹象。这种差异归因于 BCBs 的亲水性和孔径。最后，考虑了 BCBs 作为血液解毒中生物相容吸附剂的潜力。
