Liu Shih-Ju, Liao Shu-Chuan
Design and Materials for Medical Equipment and Devices, Da-Yeh University Changhua, Changhua 515006, Taiwan.
Department of Biomedical Engineering, Da-Yeh University Changhua, Changhua 515006, Taiwan.
Nanomaterials (Basel). 2021 Oct 13;11(10):2697. doi: 10.3390/nano11102697.
With the advancement of science and modern medical technology, more and more medical materials and implants are used in medical treatment and to improve human life. The safety of invasive medical materials and the prevention of infection are gradually being valued. Therefore, avoiding operation failure or wound infection and inflammation caused by surgical infection is one of the most important topics in current medical technology. Silver nanoparticles (AgNPs) have minor irritation and toxicity to cells and have a broad-spectrum antibacterial effect without causing bacterial resistance and other problems. They are also less toxic to the human body. Bamboo charcoal (BC) is a bioinert material with a porous structure, light characteristics, and low density, like bone quality. It can be used as a lightweight bone filling material. However, it does not have any antibacterial function. This study synthesized AgNPs under the ultraviolet (UV) photochemical method by reducing silver nitrate with sodium citrate. The formation and distribution of AgNPs were confirmed by UV-visible spectroscopy and X-ray diffraction measurement (XRD). The BC was treated by O plasma to increase the number of polar functional groups on the surface. Then, UV light-induced graft polymerization of N-isopropyl acrylamide (NIPAAm) and AgNPs were applied onto the BC to immobilize thermos-/antibacterial composite hydrogels on the BC surface. The structures and properties of thermos-/antibacterial composite hydrogel-modified BC surface were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared spectrum (FT-IR), and X-ray photoelectron spectroscopy (XPS). The results show that thermos-/antibacterial composite hydrogels were then successfully grafted onto BC. SEM observations showed that the thermos-/antibacterial composite hydrogels formed a membrane structure between the BC. The biocompatibility of the substrate was evaluated by Alamar Blue cell viability assay and antibacterial test in vitro.
随着科学和现代医学技术的进步，越来越多的医用材料和植入物被应用于医疗治疗和改善人类生活。侵入性医用材料的安全性和感染预防正逐渐受到重视。因此，避免手术感染导致的手术失败或伤口感染及炎症是当前医学技术中最重要的课题之一。银纳米颗粒（AgNPs）对细胞具有轻微刺激和毒性，具有广谱抗菌作用，不会引起细菌耐药性等问题，对人体的毒性也较小。竹炭（BC）是一种具有多孔结构、质轻且密度低的生物惰性材料，类似骨质，可作为轻质骨填充材料，但它没有任何抗菌功能。本研究通过用柠檬酸钠还原硝酸银，在紫外（UV）光化学方法下合成了AgNPs。通过紫外可见光谱和X射线衍射测量（XRD）确认了AgNPs的形成和分布。对BC进行O等离子体处理以增加其表面极性官能团的数量。然后，将紫外光诱导的N-异丙基丙烯酰胺（NIPAAm）和AgNPs接枝聚合应用于BC，以在BC表面固定热/抗菌复合水凝胶。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和X射线光电子能谱（XPS）对热/抗菌复合水凝胶修饰的BC表面的结构和性能进行了表征。结果表明，热/抗菌复合水凝胶随后成功接枝到BC上。SEM观察表明，热/抗菌复合水凝胶在BC之间形成了膜结构。通过Alamar Blue细胞活力测定和体外抗菌试验评估了底物的生物相容性。
