Polymer Centre, Faculty of Technology, Tomas Bata University in Zlín, T.G.M. Sq. 275, 762 72 Zlín, Czech Republic.
Colloids Surf B Biointerfaces. 2010 Jun 1;77(2):246-56. doi: 10.1016/j.colsurfb.2010.02.006. Epub 2010 Feb 11.
Medical-grade polyvinyl chloride was surface modified by a multistep physicochemical approach to improve bacterial adhesion prevention properties. This was fulfilled via surface activation by diffuse coplanar surface barrier discharge plasma followed by radical graft copolymerization of acrylic acid through surface-initiated pathway to render a structured high density brush. Three known antibacterial agents, bronopol, benzalkonium chloride, and chlorhexidine, were then individually coated onto functionalized surface to induce biological properties. Various modern surface probe techniques were employed to explore the effects of the modification steps. In vitro bacterial adhesion and biofilm formation assay was performed. Escherichia coli strain was found to be more susceptible to modifications rather than Staphylococcus aureus as up to 85% reduction in adherence degree of the former was observed upon treating with above antibacterial agents, while only chlorhexidine could retard the adhesion of the latter by 50%. Also, plasma treated and graft copolymerized samples were remarkably effective to diminish the adherence of E. coli.
医疗级聚氯乙烯通过多步物理化学方法进行表面改性，以提高抗细菌黏附性能。这是通过漫射共面表面障碍放电等离子体进行表面活化，然后通过表面引发途径进行丙烯酸自由基接枝共聚来实现的，从而形成结构化的高密度刷。然后将三种已知的抗菌剂，即布罗波尔、苯扎氯铵和洗必泰，分别涂覆到功能化表面上以诱导生物性能。采用各种现代表面探针技术来研究改性步骤的影响。进行了体外细菌黏附和生物膜形成试验。结果表明，大肠杆菌比金黄色葡萄球菌更容易受到修饰，因为在用上述抗菌剂处理后，前者的黏附程度降低了 85%，而只有洗必泰能使后者的黏附率降低 50%。此外，等离子体处理和接枝共聚样品对减少大肠杆菌的黏附非常有效。
