Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro (UFRJ),Bloco I, Ilha do Fundão, 21941-970 Rio de Janeiro, RJ, Brazil.
Res Microbiol. 2010 Oct;161(8):720-8. doi: 10.1016/j.resmic.2010.07.005. Epub 2010 Aug 7.
A peptidogalactomannan was isolated from mycelia of Cladosporium (Hormoconis) resinae and characterized using methylation-fragmentation analysis, partial acid hydrolysis and ¹H and ¹³C-NMR spectroscopy. The galactomannan component was a branched structure and consisted of a main chain containing (1→6)-linked α-d-Manp residues substituted at O-2 by side chains containing (1→2)-linked α-D-Manp residues. β-D-Galf residues were present as side chains of 3-4 units that are (1→5)-interlinked. This structure is very similar to a pGM isolated from Aspergillus fumigatus and differs from that of Cladosporium werneckii (currently named Hortaea werneckii), with this pGM and other fungal galactomannans having single terminal (1→6)-linked β-Galf residues. The importance of the carbohydrate moiety of Cladosporium resinae pGM in immunoassays was also demonstrated. On FACS examination, a decrease (60%) in rabbit serum anti- C. resinae binding to C. resinae conidia occurred when this serum had been previously incubated with pGMs from C. resinae and A. fumigatus or with mannoprotein from Candida parapsilosis, suggesting the presence of cross-reactive determinants in these fungi.
从拟茎点霉属（Hormoconis）resinae 的菌丝体中分离出一种肽半乳甘露聚糖，并通过甲基化-片段分析、部分酸水解和 ¹H 和 ¹³C-NMR 光谱进行了表征。半乳甘露聚糖成分是一种支链结构，由含有（1→6）-连接的α-d-Manp 残基的主链组成，这些残基在 O-2 处被侧链取代，侧链含有（1→2）-连接的α-D-Manp 残基。β-D-Galf 残基作为 3-4 个单元的侧链存在，这些单元通过（1→5）-键相互连接。这种结构与从烟曲霉中分离出的 pGM 非常相似，与 Cladosporium werneckii（现称为 Hortaea werneckii）的结构不同，该 pGM 与其他真菌半乳甘露聚糖具有单个末端（1→6）-连接的β-Galf 残基。还证明了拟茎点霉属 resinae pGM 中碳水化合物部分在免疫测定中的重要性。在 FACS 检查中，当用来自拟茎点霉属 resinae 和烟曲霉或近平滑念珠菌甘露糖蛋白预先孵育兔血清时，兔血清与拟茎点霉属 resinae 分生孢子的抗结合减少了 60%，表明这些真菌中存在交叉反应决定簇。
