Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité d'Epidémiologie et de Bien-Etre du Porc, B.P. 53, 22440 Ploufragan, France.
Vet Microbiol. 2011 Jan 27;147(3-4):329-39. doi: 10.1016/j.vetmic.2010.07.005. Epub 2010 Jul 15.
A longitudinal study was carried out in five French farrow-to-finish herds differently affected by respiratory diseases to describe the carrying and infection patterns of batches of sows to various respiratory pathogens during gestation and lactation. An entire batch of sows was followed during two successive reproduction cycles. Nasal, tonsillar and oro-pharyngeal swabs and blood samples were taken from each sow 9 and 4 weeks before farrowing and 1 and 4 weeks after farrowing. Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Pasteurella multocida, Haemophilus parasuis and Streptococcus suis were detected from swab samples using PCR assays. Blood samples were tested for antibodies against M. hyopneumoniae, A. pleuropneumoniae serotypes 1-9-11 and 2, Porcine Circovirus type-2 (PCV-2) and Porcine Reproductive and Respiratory Syndrome virus (PRRSV) by ELISA tests. Antibodies against H(1)N(1), H(1)N(2) and H(3)N(2) Swine Influenza Viruses (SIV) of European lineages were tested by hemagglutination inhibition assay. The results indicated that S. suis is widespread among sows (67.1% of PCR-positive sows). A. pleuropneumoniae, P. multocida, and H. parasuis were detected by PCR in 30.9%, 24.6% and 23.4% of the sows, respectively. Antibodies against M. hyopneumoniae were recovered from more than 55% of the sows in all herds whereas the micro-organism was detected in 2.4% of the sows. Although PCV-2 and SIV infections were highly prevalent, the PRRSV infection patterns ranged from no infection in farms mildly affected by respiratory diseases to active circulation in more severely affected herds. The sow population thus constitutes a reservoir for a continuous circulation of respiratory pathogens and needs to be properly considered in control strategies.
一项纵向研究在五个受呼吸道疾病影响程度不同的法国育肥-分娩场进行，旨在描述妊娠和哺乳期母猪携带和感染各种呼吸道病原体的情况。对整个批次的母猪进行了两个连续繁殖周期的跟踪。在分娩前 9 周和 4 周以及分娩后 1 周和 4 周，从每头母猪身上采集鼻拭子、扁桃体拭子和口咽拭子以及血液样本。使用 PCR 检测从拭子样本中检测到支原体肺炎、胸膜肺炎放线杆菌、多杀巴斯德菌、副猪嗜血杆菌和猪链球菌。通过 ELISA 检测血液样本中针对支原体肺炎、胸膜肺炎放线杆菌血清型 1-9-11 和 2、猪圆环病毒 2 型（PCV-2）和猪繁殖与呼吸综合征病毒（PRRSV）的抗体。通过血凝抑制试验检测针对欧洲谱系 H(1)N(1)、H(1)N(2)和 H(3)N(2)猪流感病毒（SIV）的抗体。结果表明，猪链球菌在母猪中广泛存在（67.1%的 PCR 阳性母猪）。胸膜肺炎放线杆菌、多杀巴斯德菌和副猪嗜血杆菌通过 PCR 在 30.9%、24.6%和 23.4%的母猪中检测到。所有猪场的母猪中，超过 55%的母猪检测到支原体肺炎抗体，但只有 2.4%的母猪检测到该微生物。尽管 PCV-2 和 SIV 感染非常普遍，但 PRRSV 的感染模式从呼吸道疾病轻度影响的农场没有感染到更严重影响的农场的活跃循环。因此，母猪群体是呼吸道病原体持续循环的储存库，需要在控制策略中予以适当考虑。
