Department of Chemical Engineering, National Institute of Technology, Durgapur 713209, India.
Bioresour Technol. 2010 Dec;101(24):9421-8. doi: 10.1016/j.biortech.2010.06.126. Epub 2010 Aug 8.
Papain having the characteristics of metal binding ability is immobilized on alginate bead. Design Expert Software (Version 7.1.6) uses Response Surface Methodology (RSM) for statistical designing of operating condition for immobilization of papain on alginate bead considering concentration of papain, concentration of sodium alginate, concentration of calcium chloride and pH as numeric factors and Specific Enzymatic Activity (SEA) of immobilized papain sample as response. Immobilization using 25.96 g/L papain, 20 g/L sodium alginate and 20 g/L calcium chloride at pH 7 gives the desired product as indicated by ANOVA (Analysis of Variance). Three parameters viz., initial concentration of mercury (II), amount of AIP and pH are varied in a systematic manner. Maximum 98.88% removal of mercury (II) has been achieved within 8 min when simulated aqueous solution of mercury (II) with initial concentration of 10mg/L has been contacted with 5 g of AIP at pH 9 and at 35 degrees C in a batch contactor. A mathematical model has been developed and the value of equilibrium constant for binding of mercury (II) with AIP has been found to be 126797.3.
木瓜蛋白酶具有金属结合能力的特点，被固定在海藻酸钠珠上。Design Expert Software（Version 7.1.6）使用响应面法（RSM）对固定化木瓜蛋白酶在海藻酸钠珠上的操作条件进行统计设计，考虑木瓜蛋白酶的浓度、海藻酸钠的浓度、氯化钙的浓度和 pH 值作为数值因素，固定化木瓜蛋白酶样品的比酶活（SEA）作为响应值。在 pH 值为 7 时，使用 25.96g/L 的木瓜蛋白酶、20g/L 的海藻酸钠和 20g/L 的氯化钙进行固定化，得到所需产物，这一点在方差分析（ANOVA）中得到了验证。以系统的方式改变三个参数，即初始浓度的汞（II）、AIP 的用量和 pH 值。当用初始浓度为 10mg/L 的模拟汞（II）水溶液在 pH 值为 9 和 35 摄氏度的条件下，在批式接触器中与 5g AIP 接触时，可在 8 分钟内实现高达 98.88%的汞（II）去除率。已经建立了一个数学模型，并发现 AIP 与汞（II）结合的平衡常数为 126797.3。
