Biomicrofluidics. 2010 May 24;4(2):024109. doi: 10.1063/1.3430553.
We have designed, built, and evaluated a microfluidic device that uses deterministic lateral displacement for size-based separation. The device achieves almost 100% purity and recovery in continuously sorting two, four, and six micrometer microspheres. We have applied this highly efficient device to the purification of fungal (Aspergillus) spores that are spherical ( approximately 4 mum diameter) with a narrow size distribution. Such separation directly from culture using unfiltered A. niger suspensions is difficult due to a high level of debris. The device produces a two to three increase in the ratio of spores to debris as measured by light scatter in a flow cytometer. The procedure is feasible at densities up to 4.4x10(6) sporesml. This is one of the first studies to apply microfluidic techniques to spore separations and has demonstrated that a passive separation system could significantly reduce the amount of debris in a suspension of fungal spores with virtually no loss of spore material.
我们设计、制造并评估了一种使用定向横向位移进行基于大小分离的微流控装置。该装置在连续分选两个、四个和六个微米微球时，几乎达到了 100%的纯度和回收率。我们已经将这种高效的装置应用于真菌（曲霉）孢子的纯化，这些孢子是球形的（直径约 4 微米），具有较窄的粒径分布。由于碎片含量高，直接从未经过滤的黑曲霉悬浮液中进行这种分离是很困难的。该装置通过流式细胞仪测量的光散射，使孢子与碎片的比例提高了两到三倍。在密度高达 4.4x10(6) 个孢子/ml 的情况下，该方法是可行的。这是首次将微流控技术应用于孢子分离的研究之一，证明了被动分离系统可以显著减少真菌孢子悬浮液中的碎片含量，而几乎不会损失孢子材料。
