Biomicrofluidics. 2010 May 3;4(2):024103. doi: 10.1063/1.3422524.
In this paper, a novel oscillating flow polymerase chain reaction (PCR) device was designed and fabricated to amplify SPPS150 and salmonella typhi. In this new design, the samples are shuttled (oscillating flow) inside a microfluidic chip to three different temperature zones required for DNA amplification. The amplification cycle time has markedly been reduced as the reagent volume used was only about 25% of that used in conventional PCRs. Bubble formation and adsorption issues commonly associated to chip based PCR were also eliminated. Based on the performance evaluated, it is demonstrated that this oscillating flow PCR has the advantages of both the stationary chamber and continuous flow PCR devices.
本文设计并制造了一种新型的震荡流聚合酶链反应（PCR）装置，用于扩增 SPPS150 和伤寒沙门氏菌。在这个新设计中，样品在微流控芯片内被输送（震荡流）到 DNA 扩增所需的三个不同温度区。由于使用的试剂体积仅为传统 PCR 的约 25%，因此显著缩短了扩增循环时间。也消除了基于芯片的 PCR 中常见的气泡形成和吸附问题。根据评估的性能，证明这种震荡流 PCR 结合了固定室和连续流 PCR 设备的优点。
