Biomicrofluidics. 2012 Mar;6(1):14119-1411913. doi: 10.1063/1.3690469. Epub 2012 Mar 14.
Multiple sample DNA amplification was done by using a novel rotary-linear motion polymerase chain reaction (PCR) device. A simple compact disc was used to create the stationary sample chambers which are individually temperature controlled. The PCR was performed by shuttling the samples to different temperature zones by using a combined rotary-linear movement of the disc. The device was successfully used to amplify up to 12 samples in less than 30 min with a sample volume of 5 μl. A simple spring loaded heater mechanism was introduced to enable good thermal contact between the samples and the heaters. Each of the heater temperatures are controlled by using a simple proportional-integral-derivative pulse width modulation control system. The results show a good improvement in the amplification rate and duration of the samples. The reagent volume used was reduced to nearly 25% of that used in conventional method.
采用新型旋转-直线运动聚合酶链反应（PCR）装置进行了多个样本 DNA 扩增。使用简单的光盘创建了单独控温的固定样本室。通过使用光盘的组合旋转-直线运动将样品移动到不同的温度区域来进行 PCR。该装置成功地在不到 30 分钟的时间内使用 5μl 的样品体积扩增了多达 12 个样本。引入了简单的弹簧加载加热机制，以实现样品和加热器之间的良好热接触。每个加热器温度都通过使用简单的比例积分微分脉冲宽度调制控制系统进行控制。结果表明，样品的扩增速率和持续时间有了很好的提高。所用试剂的体积减少到传统方法的近 25%。
