DNA 的介电泳：阻抗测量定量。
Dielectrophoresis of DNA: Quantification by impedance measurements.
机构信息
Fraunhofer Institute for Biomedical Engineering, D-14476 Potsdam, Germany.
出版信息
Biomicrofluidics. 2010 Jun 29;4(2):022803. doi: 10.1063/1.3430550.
Abstract
Dielectrophoretic properties of DNA have been determined by measuring capacitance changes between planar microelectrodes. DNA sizes ranged from 100 bp to 48 kbp, DNA concentrations from below 0.1 to 70 mugml. Dielectrophoretic spectra exhibited maximum response around 3 kHz and 3 MHz. The strongest response was found for very long DNA (above 10 kbp) and for short 100 bp fragments, which corresponds to the persistence length of DNA. The method allows for an uncomplicated, automatic acquisition of the dielectrophoretic properties of submicroscopical objects without the need for labeling protocols or optical accessibility.
摘要
通过测量平面微电极之间的电容变化来确定 DNA 的介电泳特性。DNA 大小范围从 100bp 到 48kbp，DNA 浓度从低于 0.1 到 70ug/ml。介电泳谱在 3kHz 和 3MHz 左右显示出最大响应。最强的响应是在非常长的 DNA（超过 10kbp）和非常短的 100bp 片段中发现的，这与 DNA 的持久长度相对应。该方法允许在不需要标记方案或光学可达性的情况下，对亚微观物体的介电泳特性进行简单、自动的获取。
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