Wu Shi-Jin, Yu Xiang, Wu Er-Miao, Chen Jian-Meng
College of Biology and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Huan Jing Ke Xue. 2010 Jun;31(6):1655-61.
The acute toxic and biochemical effects of dichloromethane and 1,2-dichloroethane to Chlorella pyrenoidosa were assessed. The experimental results showed that dichloromethane and 1, 2-dichloroethane had some inhibition to growth of the algae. With increasing concentration of the organic toxicant, the cell density decreased. The 96 h-EC50 of dichloromethane to Chlorella pyrenoidosa was 550.1 mg/L; the 96 h-EC50 of 1,2-dichloroethane to Chlorella pyrenoidosa was 276.0 mg/L, toxicity of 1,2-dichloroethane is a little stronger than that of dichloromethane; the joint toxicity of two organic toxicants was showed as antagonism. Content of chlorophyll a, activities of superoxide dismutase (SOD), catalase (CAT) and content of malondialdehyde (MDA) in algae had been significantly affected under the organic toxicant exposure. The activities of SOD and CAT were increased at first and then decreased remarkably with the increasing concentration of the organic toxicant, showed as "Bell Shaped Curve". The content of malondialdehyde (MDA) flared up and lipid peroxidant aggregated with the increasing concentration of the organic toxicant. The results suggested that the primary mechanism of two organic toxicants on Chlorella pyrenoidosa cells may be related to oxidation damage of lipid and other biological large molecules caused by dichloromethane and 1,2-dichloroethane.
评估了二氯甲烷和1,2 - 二氯乙烷对蛋白核小球藻的急性毒性和生化效应。实验结果表明，二氯甲烷和1,2 - 二氯乙烷对藻类生长有一定抑制作用。随着有机毒物浓度增加，细胞密度降低。二氯甲烷对蛋白核小球藻的96 h - EC50为550.1 mg/L；1,2 - 二氯乙烷对蛋白核小球藻的96 h - EC50为276.0 mg/L，1,2 - 二氯乙烷的毒性比二氯甲烷略强；两种有机毒物的联合毒性表现为拮抗作用。在有机毒物暴露下，藻类中叶绿素a含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性以及丙二醛（MDA）含量均受到显著影响。SOD和CAT活性随有机毒物浓度增加先升高后显著降低，呈“钟形曲线”。丙二醛（MDA）含量随有机毒物浓度增加而激增，脂质过氧化加剧。结果表明，两种有机毒物对蛋白核小球藻细胞的主要作用机制可能与二氯甲烷和1,2 - 二氯乙烷引起的脂质及其他生物大分子氧化损伤有关。
