Department of Food Science, Central Taiwan University of Science and Technology, Taichung 406, Taiwan.
Clin Chim Acta. 2010 Nov 11;411(21-22):1788-92. doi: 10.1016/j.cca.2010.07.037. Epub 2010 Aug 8.
To relate the expression of the matrix degrading proteinase cathepsin B and its endogenous inhibitor cystatin C in the synovial fluid (SF) to the clinical and laboratory variables of joint inflammation in gouty arthritis of the knee.
Thirty-nine SF samples were obtained from inflamed knees of patients with acute gout. The levels of cathepsin B, cystatin C, urokinase-type plasminogen activator (uPA), soluble uPA receptor (suPAR) and PA inhibitor type-1 (PAI-1), activities of matrix metalloproteinase-2 (MMP-2) and MMP-9, and cell counts as well as local arthritis activity scores (LAS) were examined.
The increases of cathepsin B levels correlated with increased leukocyte and neutrophil counts, latent MMP-9 (pro-MMP-9) activities, uPA, suPAR and PAI-1 levels, and uPA/PAI-1 ratios. Increased cystatin C levels corresponded closely with increased LAS, leukocyte and neutrophil counts, pro-MMP-9 activities, uPA, suPAR and PAI-1 levels, and uPA/PAI-1 ratios. Moreover, there was a correlation between cathepsin B and cystatin C levels.
These results show a high correlation between the cathepsin B/cystatin C system and markers of joint inflammation in acute gout of the knee, demonstrating the pathologic role of cathepsin B and cystatin C in inflammation.
研究膝关节痛风性关节炎滑液中基质降解蛋白酶组织蛋白酶 B 及其内源性抑制剂胱抑素 C 的表达与关节炎症的临床和实验室变量之间的关系。
从 39 例急性痛风患者的膝关节炎症滑液中获得 SF 样本。检测组织蛋白酶 B、胱抑素 C、尿激酶型纤溶酶原激活物 (uPA)、可溶性 uPA 受体 (suPAR) 和 PA 抑制剂 1 (PAI-1)、基质金属蛋白酶-2 (MMP-2) 和 MMP-9 的活性，以及细胞计数和局部关节炎活动评分 (LAS)。
组织蛋白酶 B 水平的升高与白细胞和中性粒细胞计数、潜伏 MMP-9（pro-MMP-9）活性、uPA、suPAR 和 PAI-1 水平以及 uPA/PAI-1 比值的增加相关。胱抑素 C 水平的升高与 LAS、白细胞和中性粒细胞计数、pro-MMP-9 活性、uPA、suPAR 和 PAI-1 水平以及 uPA/PAI-1 比值的增加密切相关。此外，组织蛋白酶 B 和胱抑素 C 水平之间存在相关性。
这些结果表明，膝关节急性痛风中组织蛋白酶 B/胱抑素 C 系统与关节炎症标志物之间存在高度相关性，表明组织蛋白酶 B 和胱抑素 C 在炎症中的病理作用。
