Giphart M J, Verduijn W
Department of Immunohaematology, University Hospital, Leiden, The Netherlands.
Eur J Immunogenet. 1991 Feb-Apr;18(1-2):57-68. doi: 10.1111/j.1744-313x.1991.tb00006.x.
Several sequence-specific oligonucleotides (SSO) were designed based upon available sequences of HLA-DRB1 genes which are associated with HLA-DR serotypes. Targets for these SSO were polymerase chain reaction (PCR) products using primers specific for the second exon of DRB genes. In view of practically, a hybridization system was developed which was independent of temperature or composition of the hybridization buffers, hence uniformly applicable to all SSO, yet allowing discrimination based upon one nucleotide mismatch only. The SSO typing results were compared with HLA serological typing and a high correlation was observed (99%).
基于与HLA - DR血清型相关的HLA - DRB1基因的现有序列设计了几种序列特异性寡核苷酸（SSO）。这些SSO的靶标是使用针对DRB基因第二外显子的特异性引物的聚合酶链反应（PCR）产物。考虑到实际应用，开发了一种杂交系统，该系统与杂交缓冲液的温度或组成无关，因此可统一应用于所有SSO，但仅允许基于一个核苷酸错配进行区分。将SSO分型结果与HLA血清学分型进行比较，观察到高度相关性（99%）。
