Kanchanarach Watchara, Theeragool Gunjana, Inoue Taketo, Yakushi Toshiharu, Adachi Osao, Matsushita Kazunobu
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Biosci Biotechnol Biochem. 2010;74(8):1591-7. doi: 10.1271/bbb.100183. Epub 2010 Aug 7.
Acetobacter pasteurianus strains IFO3283, SKU1108, and MSU10 were grown under acetic acid fermentation conditions, and their growth behavior was examined together with their capacity for acetic acid resistance and pellicle formation. In the fermentation process, the cells became aggregated and covered by amorphous materials in the late-log and stationary phases, but dispersed again in the second growth phase (due to overoxidation). The morphological change in the cells was accompanied by changes in sugar contents, which might be related to pellicle polysaccharide formation. To determine the relationship between pellicle formation and acetic acid resistance, a pellicle-forming R strain and a non-forming S strain were isolated, and their fermentation ability and acetic acid diffusion activity were compared. The results suggest that pellicle formation is directly related to acetic acid resistance ability, and thus is important to acetic acid fermentation in these A. pasteurianus strains.
巴氏醋酸杆菌IFO3283、SKU1108和MSU10菌株在醋酸发酵条件下培养，并检测了它们的生长行为以及耐醋酸能力和形成菌膜的能力。在发酵过程中，细胞在对数后期和稳定期聚集并被无定形物质覆盖，但在第二个生长阶段（由于过度氧化）又再次分散。细胞形态的变化伴随着糖含量的变化，这可能与菌膜多糖的形成有关。为了确定菌膜形成与耐醋酸能力之间的关系，分离出了形成菌膜的R菌株和不形成菌膜的S菌株，并比较了它们的发酵能力和醋酸扩散活性。结果表明，菌膜形成与耐醋酸能力直接相关，因此对这些巴氏醋酸杆菌菌株的醋酸发酵很重要。
