藜（灰菜）多糖对蛋鸡细胞介导免疫的调节作用
[Regulation of cellular-mediated immunity by Chenopodi boni henrici herba polysaccharides in hens].
作者信息
Hanganu Daniela, Dorhoi Anca, Pintea Adela, Olah Neli, Sevastre B
机构信息
Universitatea de Medicină şi Farmacie I. Haţieganu Cluj-Napoca Facultatea de Farmacie, Catedra de Farmacognozie, Universitatea de Stiinţe Agricole şi Medicină Veterinară Cluj-Napoca Facultatea de Medicină Veterinară.
出版信息
Rev Med Chir Soc Med Nat Iasi. 2010 Apr-Jun;114(2):587-92.
UNLABELLED
The immunomodulatory effect of polysaccharides from Chenopodium bonus-henricus herba was tested on adult hens.
MATERIAL AND METHOD
Blood samples were harvested aseptically by punction of brachial vein; ability of circulating phagocytes was assessed by the carbon particles phagocytosis test in vitro; while reactivity of peripheral lymphocytes was evaluated by lymphocytes transformation assay in vitro. Polysaharides were dissolved in saline solution and filtered throughout Millipore.
RESULTS
Phagocytic indexes (at 15 and 39 minutes) are variable according to polysaharides concentration; phagocytosis increased at 15 minutes for 40 microg/mL and 60 microg/mL, while for 30 minutes the phagocytic index decreased. In lymphocytes transformation assay, Chenopodium bonus-henricus polysaharides, alone, shown no stimulatory effect, and together to the classic mitogen, their influence was variable, but not statistical significant results were seen.
CONCLUSION
Polysaccharides have inconstant effect on phagocytosis, and mainly inhibitory role on lymphocytes proliferation.
未标记
对成年母鸡测试了藜科植物藜的多糖的免疫调节作用。
材料与方法
通过穿刺肱静脉无菌采集血样；通过体外碳颗粒吞噬试验评估循环吞噬细胞的能力；同时通过体外淋巴细胞转化试验评估外周淋巴细胞的反应性。多糖溶解于盐溶液中并通过微孔滤膜过滤。
结果
吞噬指数（在15分钟和39分钟时）根据多糖浓度而变化；对于40微克/毫升和60微克/毫升，吞噬作用在15分钟时增加，而在30分钟时吞噬指数下降。在淋巴细胞转化试验中，藜多糖单独显示无刺激作用，与经典促细胞分裂剂一起时，其影响各不相同，但未观察到统计学显著结果。
结论
多糖对吞噬作用的影响不稳定，对淋巴细胞增殖主要起抑制作用。
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