Dorhoi A, Dobrean V, Zăhan M, Virag P
Department of Infectious Diseases, Faculty of Veterinary Medicine, Cluj-Napoca, Romania.
Phytother Res. 2006 May;20(5):352-8. doi: 10.1002/ptr.1859.
Standardized ethanol extracts of Allium sativum (garlic), Glycyrrhiza glabra (licorice), Plantago major (plantain) and Hippophae rhamnoides (sea buckthorn) were assessed for their effects on cellular immunity in laying hens. Birds (n = 25) had blood samples taken and both specific and non-specific immune cell responsiveness were evaluated by a leukocyte proliferation assay, carbon clearance test and SRBC phagocytosis in monocyte-derived macrophage cultures. Licorice and sea buckthorn (50 microg/mL) clearly enhanced the macrophage membrane function (p < 0.05 and p < 0.01, respectively). Dual effects on circulating phagocytes were revealed for plantain and sea buckthorn, while garlic at 200 microg/mL impaired the phagocytic capacity of blood cells. None of the tested extracts showed mitogenic properties, but high concentrations of sea buckthorn (400 microg/mL) inhibited leukocyte proliferation. Small concentrations (20 microg/mL) of licorice proved the co-mitogenic potential for both T and B avian lymphocytes (p < 0.05). Certain extracts definitely enhanced the fowl innate and/or specific cell immunity and may therefore improve host resistance in poultry. Considering the chicken as an important non-mammalian model that also serves as an available laboratory approach for some human diseases, herbs exerting immunomodulatory properties may find relevant clinical applications.
评估了大蒜、甘草、大车前草和沙棘的标准化乙醇提取物对蛋鸡细胞免疫的影响。选取25只鸡采集血样，通过白细胞增殖试验、碳清除试验以及单核细胞衍生巨噬细胞培养中的SRBC吞噬作用来评估特异性和非特异性免疫细胞反应性。甘草和沙棘（50微克/毫升）明显增强了巨噬细胞膜功能（分别为p < 0.05和p < 0.01）。大车前草和沙棘对循环吞噬细胞有双重作用，而200微克/毫升的大蒜损害了血细胞的吞噬能力。所测试的提取物均未显示有丝分裂特性，但高浓度的沙棘（400微克/毫升）抑制白细胞增殖。低浓度（20微克/毫升）的甘草证明对禽类T和B淋巴细胞具有协同有丝分裂潜能（p < 0.05）。某些提取物确实增强了家禽的先天性和/或特异性细胞免疫，因此可能提高家禽的宿主抵抗力。鉴于鸡作为一种重要的非哺乳动物模型，也可作为研究某些人类疾病的可用实验室方法，具有免疫调节特性的草药可能会有相关的临床应用。
